Lanostane-Type Saponins from .

The phytochemistry of the genera , , and , belonging to the Primulaceae family is not well studied so far. Hence, in this paper, we present the results of UHPLC-MS/MS analysis of several primrose family members as well as isolation and structure determination of two new saponins from subsp. . These two -triterpenoid saponins were characterized as (23)-17α,23-epoxy-29-hydroxy-3β-[(-β-d-glucopyranosyl-(1→2)--α-l-rhamnopyranosyl-(1→2)--β-d-glucopyranosyl-(1→2)--α-l-arabinopyranosyl-(1→6)-β-d-glucopyranosyl)oxy]-27--lanost-8-en-25-one and (23)-17α,23-epoxy-29-hydroxy-3β-[(-α-l-rhamnopyranosyl-(1→2)--β-d-glucopyranosyl-(1→2)--α-l-arabinopyranosyl-(1→6)-β-d-glucopyranosyl)oxy]-27--lanost-8-en-25-one, respectively. Their structures were determined by high resolution mass spectrometry (HRMS), tandem mass spectrometry (MS/MS), one- and two-dimensional nuclear magnetic resonance spectroscopy (1D-, and 2D-NMR) analyses. So far, the 27--lanostane monodesmosides were rarely found in dicotyledon plants. Therefore their presence in and also in species belonging to the section is unique and reported here for the first time. Additionally, eleven other saponins were determined by HRMS and MS/MS spectra. The isolated lanostane saponins can be considered as chemotaxonomic markers of the family Primulaceae.