Mutations of the Integrator subunits are associated with neurodevelopmental disorders and cancers. However, their role during neural development is poorly understood. Here, we demonstrate that the Drosophila Integrator complex prevents dedifferentiation of intermediate neural progenitors (INPs) during neural stem cell (neuroblast) lineage development. Loss of intS5, intS8, and intS1 generated ectopic type II neuroblasts. INP-specific knockdown of intS8, intS1, and intS2 resulted in the formation of excess type II neuroblasts, indicating that Integrator prevents INP dedifferentiation. Cell-type-specific DamID analysis identified 1413 IntS5-binding sites in INPs, including zinc-finger transcription factor earmuff (erm). Furthermore, erm expression is lost in intS5 and intS8 mutant neuroblast lineages, and intS8 genetically interacts with erm to suppress the formation of ectopic neuroblasts. Taken together, our data demonstrate that the Drosophila Integrator complex plays a critical role in preventing INP dedifferentiation primarily by regulating a key transcription factor Erm that also suppresses INP dedifferentiation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.celrep.2019.03.089 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Using Evidence-Based Practice in the Transition to Telepractice: Case Study of a Complexity-Based Speech Sound Intervention.
Perspect ASHA Spec Interest Groups
August 2023
School of Speech, Language, and Hearing Sciences, San Diego State University, San Diego, CA.
Purpose: Speech-language pathologists (SLPs) are tasked with integrating the principles of evidence-based practice (EBP) to provide effective and efficient assessment and intervention services that best support clients and their families. As new research, technologies, and perspectives emerge, SLPs are required to adapt their clinical practices to meet these changes while maintaining high-quality evidence-based services. Through an illustrative case study, we aim to demonstrate the process of applying EBP principles - including research evidence, client and family perspectives, and clinical expertise - to a complexity-based speech sound intervention delivered via telepractice.
View Article and Find Full Text PDF"Enhancing equitable impact: ethical, legal, and sustainable approaches in short-term surgical outreach for global child health".
Front Pediatr
January 2025
Department of Anesthesiology, University of Wisconsin Foundation, Madison, WI, United States.
Global health prioritizes improving health and achieving equity in health for all people worldwide. It encompasses a wide range of efforts, including disease prevention and treatment, health promotion, healthcare delivery, and addressing health disparities across borders. Short-term medical and surgical missions often contribute to the global health landscape, especially in low and lower-middle income countries.
View Article and Find Full Text PDFComputational identification of novel natural inhibitors against triple mutant DNA gyrase A in fluoroquinolone-resistant Typhimurium.
Biochem Biophys Rep
March 2025
Department of Integrative Biology, School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore, 632014, Tamil Nadu, India.
The rising resistance to fluoroquinolones in Typhimurium poses a significant global health challenge. This computational research addresses the pressing need for new therapeutic drugs by utilizing various computational tools to identify potential natural compounds that can inhibit the triple mutant DNA gyrase subunit A enzyme, which is crucial in fluoroquinolone resistance. Initially, the three-dimensional structure of the wild-type DNA gyrase A protein was modeled using homology modeling, and followed by mutagenesis to create the clinically relevant triple mutant (SER83PHE, ASP87GLY, ALA119SER) DNA gyrase A protein structure.
View Article and Find Full Text PDFDepression-related innate immune genes and pan-cancer gene analysis and validation.
Front Genet
January 2025
Department of Pharmacology, The Key Laboratory of Neural and Vascular Biology, The Key Laboratory of New Drug Pharmacology and Toxicology, Ministry of Education, Collaborative Innovation Center of Hebei Province for Mechanism, Diagnosis and Treatment of Neuropsychiatric Diseases, Hebei Medical University, Shijiazhuang, Hebei, China.
Background: Depression, a prevalent chronic mental disorder, presents complexities and treatment challenges that drive researchers to seek new, precise therapeutic targets. Additionally, the potential connection between depression and cancer has garnered significant attention.
Methods: This study analyzed depression-related gene expression data from the GEO database.
Landscapes-a lens for assessing sustainability.
Landsc Ecol
January 2025
Department of Geography, McGill University, Montreal, QC Canada.
Context: There are urgent calls to transition society to more sustainable trajectories, at scales ranging from local to global. Landscape sustainability (LS), or the capacity for landscapes to provide equitable access to ecosystem services essential for human wellbeing for both current and future generations, provides an operational approach to monitor these transitions. However, the complexity of landscapes complicates how and what to consider when assessing LS.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!