AI Article Synopsis
- MicroRNAs, particularly miR-144, play a significant role in the resistance of cervical cancer cells to cisplatin (CDDP) treatment.
- The study demonstrated that overexpression of miR-144 leads to reduced cell viability, increased apoptosis, and inhibited migration and invasion in cervical cancer cells resistant to CDDP.
- miR-144 directly targets the LHX2 gene, and restoring LHX2 expression counteracts the pro-apoptotic and anti-invasive effects of miR-144, suggesting that miR-144 can help overcome CDDP resistance in cervical cancer by affecting LHX2 levels.
Article Abstract
Mounting evidence showed that microRNAs involve in development and chemoresistance of various human cancers. We explored the roles and mechanisms of miR-144 in resistance to cisplatin (CDDP) of cervical cancer cells. miR-144 and LIM homeobox 2 (LHX2) expression in CDDP-resistant and the parental cells was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis, respectively. The functions of miR-144 overexpression on cell viability, the incidence of apoptosis, the activity of caspase-3/7, the cleaved-caspase-3 expression, cell migration, and invasion were determined in Hela cells and Hela/CDDP cells. Overexpression of miR-144 reduced cell viability, induced cell apoptosis, and inhibited cell migration and invasion after CDDP treatment. Besides, a luciferase reporter system demonstrated that miR-144 could directly bind to the 3' untranslated region (3'-UTR) of LHX2 messenger RNA (mRNA). Gain expression of miR-144 decreased the expression of LHX2 both in mRNA and protein levels. Furthermore, restoration of LHX2 partly abolished the biological functions of miR-144 in resistance of cervical cancer cells. Taken together, miR-144 overcomes resistance to CDDP via promoting cell apoptosis and inhibiting invasion through targeting LHX2 in cervical cancer cells.
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