The objective of this study was to screen and identify rickettsial organisms in ectoparasites collected from dogs in a shelter in Gomez Palacio, Durango, Mexico. One hundred dogs were inspected for ectoparasites. All the dogs were parasitized with Rhipicephalus sanguineus ticks, three with Heterodoxus spiniger lice and one with Ctenocephalides felis fleas. DNA was extracted from the ectoparasites found on each dog, and PCR with the primers for the Anaplasmataceae 16S rRNA and citrate synthase gltA genes were performed. Eight DNA samples obtained from ticks, three from lice and one from fleas were positive to 16S rRNA. Only one sample from C. felis and one from H. spiniger were positive to gltA. Sequence analysis of amplified products from C. felis showed identity to Rickettsia felis, Wolbachia pipientis, and Wolbachia spp., while a sequence from H. spiniger showed identity to Wolbachia spp. Herein we report the molecular detection of R. felis, W. pipientis, and Wolbachia spp. in C. felis and H. spiniger in northern Mexico. These results contribute to the knowledge of the microorganisms present in ectoparasites from dogs in Mexico.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.vprsr.2017.10.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular evidence of Wolbachia in bat-associated mite Periglischrus Iheringi Oudemans, 1902 (Mesostigmata: Spinturnicidae) from Brazil.
Vet Res Commun
December 2024
Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Wolbachia is an intracellular endosymbiont bacterium found in nematodes and arthopods. Regarding mites, the Wolbachia supergroup U has been described based on strains found in the genus Spinturnix. In this study, ten specimens of Periglischrus iheringi (Mesostigmata: Spinturnicidae), collected from Artibeus obscurus (Chiroptera: Phyllostomidae) in Santa Catarina State, were found to be infected with Wolbachia.
View Article and Find Full Text PDFPopulation dynamics of sympatric Phortica spp. and first record of stable presence of Phortica oldenbergi in a Thelazia callipaeda-endemic area of Italy.
Parasit Vectors
November 2024
Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Rome, Italy.
Article Synopsis
- The study focuses on five species of Phortica flies in Europe and the Middle East, particularly Phortica variegata and Phortica okadai, known as vectors for the zoonotic eyeworm Thelazia callipaeda, in a region near Rome, Italy.
- Over three years (2018-2020), researchers collected and identified nearly 5,600 flies, revealing a dominance of Phortica variegata, which was found to be infected with T. callipaeda, while Phortica oldenbergi showed differing behavior in fruit traps.
- Environmental factors like temperature, wind speed, and pressure influenced the population dynamics of these species, with the study being the first to explore these ecological interactions
Discovery of a novel in expands nematode host distribution.
Front Microbiol
September 2024
Department of Biological Sciences, Texas Tech University, Lubbock, TX, United States.
Bioinformatics sequence data mining can reveal hidden microbial symbionts that might normally be filtered and removed as contaminants. Data mining can be helpful to detect , a widespread bacterial endosymbiont in insects and filarial nematodes whose distribution in plant-parasitic nematodes (PPNs) remains underexplored. To date, has only been reported a few PPNs, yet nematode-infecting may have been widespread in the evolutionary history of the phylum based on evidence of horizontal gene transfers, suggesting there may be undiscovered infections in PPNs.
View Article and Find Full Text PDFDiversity of Anaplasmataceae Transmitted by Ticks (Ixodidae) and the First Molecular Evidence of and Anaplasma boleense in Paraguay.
Microorganisms
September 2024
Center for the Development of Scientific Research (CEDIC), Manduvira 635, Asunción 1255, Paraguay.
Anaplasmataceae bacteria are emerging infectious agents transmitted by ticks. The aim of this study was to identify the molecular diversity of this bacterial family in ticks and hosts, both domestic and wild, as well as blood meal sources of free-living ticks in northeastern Paraguay. The bacteria were identified using PCR-HRM, a method optimized for this purpose, while the identification of ticks and their blood meal was performed using conventional PCR.
View Article and Find Full Text PDFMicrobial Community Structure and Metabolic Function in the Venom Glands of the Predatory Stink Bug, (Hemiptera: Pentatomidae).
Insects
September 2024
Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.
The predatory stink bug, (Hemiptera: Pentatomidae), is an important and valuable natural enemy of insect pests in their ecosystems. While insects are known to harbor symbiotic microorganisms, and these microbial symbionts play a crucial role in various aspects of the host's biology, there is a paucity of knowledge regarding the microbiota present in the venom glands of . This study investigated the venom glands of adult bugs using both traditional in vitro isolation and cultural methods, as well as Illumina high-throughput sequencing technology.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!