Microorganisms can be genetically engineered to solve a range of challenges in diverse including health, environmental protection and sustainability. The natural complexity of biological systems makes this an iterative cycle, perturbing metabolism and making stepwise progress toward a desired phenotype through four major stages: design, build, test, and data interpretation. This cycle has been accelerated by advances in molecular biology (e.g. robust DNA synthesis and assembly techniques), liquid handling automation and scale-down characterization platforms, generating large heterogeneous data sets. Here, we present an extensible Python package for scientists and engineers working with large biological data sets to interpret, model, and visualize data: the IMPACT (Integrated Microbial Physiology: Analysis, Characterization and Translation) framework. Impact aims to ease the development of Python-based data analysis workflows for a range of stakeholders in the bioengineering process, offering open-source tools for data analysis, physiology characterization and translation to visualization. Using this framework, biologists and engineers can opt for reproducible and extensible programmatic data analysis workflows, mediating a bottleneck limiting the throughput of microbial engineering. The Impact framework is available at https://github.com/lmse/impact.
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http://dx.doi.org/10.1016/j.mec.2019.e00089 | DOI Listing |
Hum Exp Toxicol
January 2025
Department of Gynecology and Obstetrics, Fuyong People's Hospital, Shenzhen, China.
Gestational diabetes mellitus (GDM) is a metabolic disorder that arises during pregnancy and heightens the risk of placental dysplasia. Ginsenoside Re (Re) may stabilize insulin and glucagon to regulate glucose levels, which may improve diabetes-associated diseases. This study aims to investigate the mechanism of Re in high glucose (HG)-induced apoptosis of trophoblasts through endoplasmic reticulum stress (ERS)-related protein CHOP/GADD153.
View Article and Find Full Text PDFJ Gerontol B Psychol Sci Soc Sci
January 2025
Linguistics and English as a Second Language, Faculty of Arts, University of Groningen, Groningen, the Netherlands.
Objectives: The complex life experience of speaking two or more languages has been suggested to preserve cognition in older adulthood. This study aimed to investigate this further by examining the relationship between multilingual experience variables and cognitive functioning in a large cohort of older adults in the diversely multilingual north of the Netherlands.
Method: 11,332 older individuals participating in the Lifelines Cohort Study completed a language experience questionnaire.
Mol Diagn Ther
January 2025
Istituto Europeo di Oncologia, IRCCS, Via Adamello 16, 20139, Milan, Italy.
Background: Predicting response to targeted cancer therapies increasingly relies on both simple and complex genetic biomarkers. Comprehensive genomic profiling using high-throughput assays must be evaluated for reproducibility and accuracy compared with existing methods.
Methods: This study is a multicenter evaluation of the Oncomine™ Comprehensive Assay Plus (OCA Plus) Pan-Cancer Research Panel for comprehensive genomic profiling of solid tumors.
Am J Cardiovasc Drugs
January 2025
Division of Cardiology, Department of Internal Medicine, Ilsan Paik Hospital, Inje University College of Medicine, Goyang, Republic of Korea.
Background: Amiodarone is an effective anti-arrhythmic drug; however, it is frequently associated with thyroid dysfunction. The aim of this study was to investigate the incidence and risk factor of amiodarone-induced dysfunction in an iodine-sufficient area.
Methods: This retrospective cohort study included 27,023 consecutive patients treated with amiodarone for arrhythmia, using the Korean National Health Insurance database.
Int Urol Nephrol
January 2025
Department of Colorectal Surgery, Heliopolis Hospital, São Paulo, SP, Brazil.
Purpose: Locally advanced colorectal tumors frequently invade adjacent organs, particularly the urinary bladder in the sigmoid colon and upper rectum, complicating multivisceral resections. This study compared postoperative outcomes of partial cystectomy (PC) and total cystectomy (TC) in patients with locally advanced colorectal cancer.
Methods: A systematic review was conducted in PubMed, Scopus, Central Register of Clinical Trials, and Web of Science for studies published up to November 2024.
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