Large-eddy simulations of thermal convection are presented and discussed for a cube with rough horizontal surfaces. Two types of roughness are considered: uniformly placed pyramids, and grooves aligned parallel to one set of sidewalls. The Rayleigh number is 10^{8}, the Prandtl number 0.7, and the aspect ratio 1, as in a previous study [N. Foroozani, J. J. Niemela, V. Armenio, and K. R. Sreenivasan, Phys. Rev. E 95, 033107 (2017)10.1103/PhysRevE.95.033107], except that the meshes here are finer. When the thermal boundary layers are sufficiently large relative to the characteristic roughness height, i.e., for hydrodynamically smooth conditions, the mean properties of the large scale circulation (LSC) are qualitatively similar to the case of smooth surfaces. In particular, the LSC is always aligned along one of the diagonals of the cube. When the boundaries are hydrodynamically rough, the same result holds true only for the case of pyramidal structures; for grooved surfaces, the LSC is forced to be parallel to the sidewalls on average, alternating rapidly between the two diagonals of the cube with a mean period of the order 10 turnover times. Our analysis suggests that the difference from the pyramidal case is due to the breaking of the horizontal x-z symmetry under conditions of hydrodynamical roughness, and the corresponding directional concentration of plume emission along the grooves, from which the LSC is generated, providing a strong restoring force. Furthermore, in this study we observed a small reduction in heat transport for both roughness configurations which is in good agreement with past studies.
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ACS Sens
January 2025
Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Solid-phase immunosorbent reactions, such as ELISA, are widely used for detecting, identifying, and quantifying protein markers. However, traditional centimeter scale well-based immunoreactors suffer from low surface-to-volume (S/V) ratios, leading to large sample consumption and a long assay time. Microfluidic technologies, particularly tubular microfluidic immunoreactors, have emerged as promising alternatives due to their high S/V ratios.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
February 2025
State Key Laboratory of Genetic Engineering, School of Life Sciences, Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200438, China.
Aging is a complex process that affects multiple organs, and the discovery of a pharmacological approach to ameliorate aging is considered the Holy Grail of medicine. Here, we performed an N-ethyl-N-nitrosourea forward genetic screening in zebrafish and identified an accelerated aging mutant named (), harboring a mutation in the - () gene. Loss of leads to a short lifespan and age-related characteristics in the intestine of zebrafish embryos, such as cellular senescence, genomic instability, and epigenetic alteration.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
February 2025
Museum of Natural History, University of Colorado-Boulder, Boulder, CO 80309.
Amid global challenges like climate change, extinctions, and disease epidemics, science and society require nuanced, international solutions that are grounded in robust, interdisciplinary perspectives and datasets that span deep time. Natural history collections, from modern biological specimens to the archaeological and fossil records, are crucial tools for understanding cultural and biological processes that shape our modern world. At the same time, natural history collections in low and middle-income countries are at-risk and underresourced, imperiling efforts to build the infrastructure and scientific capacity necessary to tackle critical challenges.
View Article and Find Full Text PDFScience
January 2025
Department of Genetics, School of Medicine, Stanford University, Stanford, CA, USA.
Large genome rearrangements in mammalian cells can be generated at scale.
View Article and Find Full Text PDFWe lack tools to edit DNA sequences at scales necessary to study 99% of the human genome that is noncoding. To address this gap, we applied CRISPR prime editing to insert recombination handles into repetitive sequences, up to 1697 per cell line, which enables generating large-scale deletions, inversions, translocations, and circular DNA. Recombinase induction produced more than 100 stochastic megabase-sized rearrangements in each cell.
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