Molecular Mapping of Stripe Rust Resistance Gene in a Common Wheat Landrace Aus27430.

Plant Dis

1 The University of Sydney Plant Breeding Institute, School of Life and Environment Sciences, Faculty of Science, Cobbitty, NSW 2570, Australia.

Published: June 2019

The deployment of diverse sources of resistance in new cultivars underpins durable control of rust diseases. Aus27430 exhibited a moderate level of stripe rust resistance against f. sp. (Pst) pathotypes currently prevalent in Australia. Aus27430 was crossed with the susceptible parent Avocet S (AvS) and subsequent filial generations were raised. Monogenic segregation observed among Aus27430/AvS F families was confirmed through stripe rust screening of an F recombinant inbred line (RIL) population, and the resistance locus was temporarily named . Selective genotyping using an Illumina iSelect 90K wheat SNP bead chip array located in chromosome 6A. Genetic mapping of the RIL population with linked 90K SNPs that were converted into PCR-based marker assays, as well as SSR markers previously mapped to chromosome 6A, confirmed the chromosomal assignment for . Comparative analysis of other stripe rust resistance genes located in chromosome 6A led to the formal designation of as . Tests with a marker linked with also demonstrated the presence of this gene in Aus27430. interacted with to produce stripe rust responses lower than those produced by RILs carrying these genes individually. Although showed higher recombination with compared with the other flanking marker _, it amplified the AvS allele in 80 cultivars, whereas amplified AvS allele in 67 cultivars. Both markers can be used in marker-assisted selection after confirming parental polymorphism.

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-06-18-1055-REDOI Listing

Publication Analysis

Top Keywords

stripe rust
20
rust resistance
12
ril population
8
located chromosome
8
amplified avs
8
avs allele
8
allele cultivars
8
rust
6
stripe
5
resistance
5

Similar Publications

Re-Examination Characterization and Screening of Stripe Rust Resistance Gene of Wheat Gene Family Based on the Transcriptome in Xinchun 32.

Int J Mol Sci

January 2025

Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China.

Pathogenesis-related protein-1 (PR1) encodes a water-soluble protein produced in plants after pathogen infection or abiotic stimulation. It plays a crucial role in plant-induced resistance by attacking pathogens, degrading cell wall macromolecules and pathogen toxins, and inhibiting the binding of viral coat proteins to plant receptor molecules. Compared to model plants, the mechanism of action of PR1 in wheat remains underexplored.

View Article and Find Full Text PDF

Background: Puccinia striiformis f. sp. tritici (Pst) causes wheat stripe (yellow) rust disease, which is one of the most destructive diseases affecting wheat worldwide.

View Article and Find Full Text PDF

Wheat stripe rust caused by the fungus Puccinia striiformis f. sp. tritici (Pst) is currently the most destructive disease of wheat.

View Article and Find Full Text PDF

Stripe rust, induced by f. sp. (), is one of the most destructive fungal diseases of wheat worldwide.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!