The inclusion of special regulatory sequences known as matrix attachment regions (MARs) in transgene constructs has been suggested as a possible approach to stabilise the expression of foreign heterological genes. The present review provides a brief summary regarding the MARs that have been used in investigations studying their influence on plant transgene expression in different plants with different promoters and reporter genes, and the comparison of these investigations.
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http://dx.doi.org/10.1007/s13205-019-1709-5 | DOI Listing |
Int J Mol Sci
December 2024
Department of Physiology, Faculty of Medicine, Semmelweis University, 37-47 Tűzoltó Street, 1094 Budapest, Hungary.
The octapeptide angiotensin II (Ang II) is a circulating hormone as well as a locally formed agonist synthesized by the angiotensin-converting enzyme (ACE) of endothelial cells. It forms a powerful mechanism to control the amount and pressure of body fluids. All main effects are directed to save body salt and water and ensure blood pressure under basic conditions and in emergencies.
View Article and Find Full Text PDFPolymers (Basel)
December 2024
Institute of Chemistry, Federal University of Uberlândia, Uberlândia 38400-902, Brazil.
Cellulose tosylate (MCC-Tos) is a key derivative for surface modification and a crucial precursor for cellulose compatibilization in click reactions, enabling its functionalization for advanced applications. Replacing tosyl groups with alkyne groups broadens cellulose's potential in biocompatible reactions, such as thiol-yne click chemistry and protein/enzyme immobilization. To achieve this, we optimized the heterogeneous synthesis of MCC-Tos using a Doehlert matrix statistical design, evaluating the influence and interaction of the reaction conditions.
View Article and Find Full Text PDFACS Appl Bio Mater
January 2025
School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Bone is a dynamic tissue that serves several purposes in the human body, including storing calcium, forming blood cells, and protecting and supporting the body's organs. Alkaline phosphatase (ALP) is secreted into the circulation by osteoblasts, the cells responsible for making bone. It attaches to the surface of osteoblast cells or matrix vesicles.
View Article and Find Full Text PDFJ Biomed Mater Res A
January 2025
PRISM Research Institute, Technological University of the Shannon: Midlands Midwest, Athlone, Ireland.
This study provides a comprehensive investigation of antimicrobial additives (ZnO/AgNPs and SiO/AgNPs) on the properties of biodegradable ternary blends composed of poly(hydroxybutyrate) (PHB), poly(lactic acid) (PLA), and polycaprolactone (PCL) by examining the morphology, thermal stability, crystallinity index, and cell viability of these blends. Overall, transmission electron microscopy (TEM) analysis revealed that AgNPs and SiO exhibited comparable sizes, whereas ZnO was significantly larger, which influences their release profiles and interactions with the blends. The addition of antimicrobials influences the rheology of the blends, acting as compatibilizers by reducing the intermolecular forces between biopolymers.
View Article and Find Full Text PDFCell Mol Biol Lett
January 2025
Department of Molecular Biology, Ruđer Bošković Institute, 10000, Zagreb, Croatia.
Proper adhesion of cells to their environment is essential for the normal functioning of single cells and multicellular organisms. To attach to the extracellular matrix (ECM), mammalian cells form integrin adhesion complexes consisting of many proteins that together link the ECM and the actin cytoskeleton. Similar to mammalian cells, the amoeboid cells of the protist Dictyostelium discoideum also use multiprotein adhesion complexes to control their attachment to the underlying surface.
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