AI Article Synopsis
- Researchers developed methods to conjugate DNA with proteins, enhancing the integration of their functions.
- They used protein-binding peptides to direct a DNA reaction, creating DNA-protein conjugates effectively, even in complex environments like cell lysates.
- A star-shaped DNA nanostructure was utilized to assemble multiple DNA-Rituximab conjugates into a pseudo-IgM antibody, which was analyzed using electron microscopy techniques.
Article Abstract
The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein-binding peptides can direct a DNA-templated reaction, selectively furnishing DNA-protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star-like pentameric DNA nanostructure to assemble five DNA-Rituximab conjugates, made by our reported method, into a pseudo-IgM antibody structure that was subsequently characterized by negative-stain transmission electron microscopy (nsTEM) analysis.
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| http://dx.doi.org/10.1002/anie.201903134 | DOI Listing |
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Peptide-Directed DNA-Templated Protein Labelling for The Assembly of a Pseudo-IgM.
Angew Chem Int Ed Engl
July 2019
Department of Chemistry and Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus, Denmark.
Article Synopsis
- Researchers developed methods to conjugate DNA with proteins, enhancing the integration of their functions.
- They used protein-binding peptides to direct a DNA reaction, creating DNA-protein conjugates effectively, even in complex environments like cell lysates.
- A star-shaped DNA nanostructure was utilized to assemble multiple DNA-Rituximab conjugates into a pseudo-IgM antibody, which was analyzed using electron microscopy techniques.
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