Dynamics of industrial biosynthesis of polyenzymic Streptomyces griseus system proteases and changes in their activities are studied in the process of streptomycin production. The method to isolate protease preparations is developed with optimization of production cycles of the stabilization, vacuum-concentration, ballast protein salting-out and lyophilization stages. The preparation is low toxic, retains 98% of the proteolytic activity at the temperature of 60 degrees C, 30 and 80%--under the effect of EDTA and urea, the activity maximum is at pH 7.0-8.0. The production technique of the preparation is estimated for its economic efficiency.
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J Fungi (Basel)
January 2025
Joint Vietnam-Russia Tropical Science and Technology Research Center, Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi 122100, Vietnam.
The diversity and resource potential of macroscopic fungi in tropical regions remain understudied. Vietnam, being in a biodiversity hotspot, has a large number of new fungal species that are of interest for biotechnology and medicine. The presence of a large number of protected areas in Vietnam creates favorable opportunities for the study and ex situ conservation of tropical biodiversity.
View Article and Find Full Text PDFFood Res Int
February 2025
Food Science and Nutrition Department, University of Minnesota, 1334 Eckles Ave, Saint Paul, MN, 55108, United States. Electronic address:
There is an ever-increasing demand for novel plant proteins that are non-allergenic, nutritionally complete, adequately functional, and can be sustainably sourced. RuBisCo is a protein that fulfills these requirements and can be sourced from alfalfa (Medicago sativa). Therefore, this study investigated several techniques to adequately extract alfalfa protein.
View Article and Find Full Text PDFSci Total Environ
January 2025
School of Chemical and Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Thanjavur 613 401, Tamil Nadu, India. Electronic address:
Microbially Induced Calcium Carbonate Precipitation (MICP) plays a significant role in coastal soil stabilization and erosion prevention. In the present study, the biomineralizing potential of a newly isolated Bacillus sp. N₉ was investigated through MICP.
View Article and Find Full Text PDFPLoS One
January 2025
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Tigecycline (Tgc), a third-generation tetracycline is found as the last line of defense against multi-drug resistant bacteria. Recent increased rate of resistance to tgc, a human-restricted agent among animal bacteria poses a significant global health challenge. Overuse of first generation tetracyclines (Tet) and phenicols in animals have been suggested to be associated with Tgc resistance development.
View Article and Find Full Text PDFCurr Microbiol
January 2025
Unit of Microbiology and Immunology, ICMR-Vector Control Research Centre, Medical Complex, Indira Nagar, Puducherry, 605006, India.
In recent years, there has been a global threat from emerging vector-borne diseases (VBD), despite the implementation of several vector control programs. Considering the benefits of bacterial pesticides, the present study aimed to isolate potential mosquitocidal bacteria from the various soil types collected from the Kasaragod (12.5°N, 75.
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