Daily Variations in the Expression of Genes Related to Insecticide Resistance in the Chagas Disease Vector (Hemiptera: Reduviidae).

Pyrethroid resistance has been detected in , which in part has been attributed to increased oxidative metabolism of the insecticide by cytochrome P450s. Nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) catalyzes electron transfer from NADPH to all known cytochrome P450s. In this study, the expression of the gene at transcriptional level was determined in different tissues and two life stages. The expression patterns showed differences in the tissues and stages studied, suggesting differential metabolic requirements. On the other hand, to investigate the presence of rhythms in the expression of genes related with insecticide resistance, we explored the daily expression profile of the gene and a gene () in fat body from adults of under different dark/light regimes. The results suggest that gene expression is under endogenous clock regulation and show a rhythmic profile in the expression of the gene.