The performance of the GENE-TRAK Salmonella assay in food as been evaluated with samples of flour or flour based bakery mix provided by the American Association of Cereal Chemists (AACC) check sample program. Six pairs of coded samples were tested from November 1986 through September 1987. The test correctly identified all six positive and six negative samples. This included a lactose positive Salmonella strain that was missed by a large majority of the other laboratories that participated in the survey. These results indicate that DNA probe technology can provide not only more timely results than traditional methods, but in some cases can actually provide more accurate information.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028X-51.7.579 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Automated Library Construction and Analysis for High-Throughput Nanopore Sequencing of SARS-CoV-2.
J Appl Lab Med
September 2022
Canada's Michael Smith Genome Sciences Centre at BC Cancer, Vancouver, BC, Canada.
Background: To support the implementation of high-throughput pipelines suitable for SARS-CoV-2 sequencing and analysis in a clinical laboratory, we developed an automated sample preparation and analysis workflow.
Methods: We used the established ARTIC protocol with approximately 400 bp amplicons sequenced on Oxford Nanopore's MinION. Sequences were analyzed using Nextclade, assigning both a clade and quality score to each sample.
Molecular characterization and combined genotype association study of bovine cluster of differentiation 14 gene with clinical mastitis in crossbred dairy cattle.
Vet World
July 2016
Molecular Genetics Laboratory, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, Haryana, India.
Aim: The present study was undertaken with the objectives to characterize and to analyze combined genotypes of cluster of differentiation 14 (CD14) gene to explore its association with clinical mastitis in Karan Fries (KF) cows maintained in the National Dairy Research Institute herd, Karnal.
Materials And Methods: Genomic DNA was extracted using blood of randomly selected 94 KF lactating cattle by phenol-chloroform method. After checking its quality and quantity, polymerase chain reaction (PCR) was carried out using six sets of reported gene-specific primers to amplify complete KF CD14 gene.
Computer-aided clinical laboratory diagnosis in conjunction with the electronic medical textbook.
Medinfo
April 1996
Diagnostics Division, Otsuka Pharmaceutical Co. LTD., 224-18 Hiraishi, Kawauchi-cho, Tokushima, Japan.
1. INTRODUCTION. Medical knowledge has been increasing and diversifying on a worldwide scale, while the specialization of physicians has been extended vigorously.
View Article and Find Full Text PDFSimultaneous liquid chromatographic determination of thiamine and riboflavin in selected foods.
J AOAC Int
January 1994
Waters Chromatography Division of Millipore, Milford, MA 01757.
A reliable, improved liquid chromatographic (LC) method has been developed for the measurement of thiamine and riboflavin in foods. The major improvement in the method is the chromatographic separation achieved. The method is also very reproducible and extremely sensitive.
View Article and Find Full Text PDFPerformance of a DNA Probe-Based Salmonella Test in the AACC Check Sample Program.
J Food Prot
July 1988
GENE-TRAK Systems, Framingham, Massachusetts 01701.
The performance of the GENE-TRAK Salmonella assay in food as been evaluated with samples of flour or flour based bakery mix provided by the American Association of Cereal Chemists (AACC) check sample program. Six pairs of coded samples were tested from November 1986 through September 1987. The test correctly identified all six positive and six negative samples.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!