Comparison of cell mechanical measurements provided by Atomic Force Microscopy (AFM) and Micropipette Aspiration (MPA).

J Mech Behav Biomed Mater

Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain; CIBER-BBN, Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine, Spain.

Published: July 2019

AI Article Synopsis

  • A comparative study of T-lymphocyte mechanical properties using Micropipette Aspiration (MPA) and Atomic Force Microscopy (AFM) reveals significant differences in elastic modulus values due to the methodologies used.
  • The initial fitting of data to Hertz and Theret models showed non-Gaussian distributions, while refined models like the simplified double contact and Zhou models resulted in reduced discrepancies in elastic modulus calculations.
  • The analysis suggests that the localized measurements from AFM versus the broader scope of MPA may explain these variations in results.

Article Abstract

A comparative analysis of T-lymphocyte mechanical data obtained from Micropipette Aspiration (MPA) and Atomic Force Microscopy (AFM) is presented. Results obtained by fitting the experimental data to simple Hertz and Theret models led to non-Gaussian distributions and significantly different values of the elastic moduli obtained by both techniques. The use of more refined models, taking into account the finite size of cells (simplified double contact and Zhou models) reduces the differences in the values calculated for the elastic moduli. Several possible sources for the discrepancy between the techniques are considered. The analysis suggests that the local nature of AFM measurements compared with the more general character of MPA measurements probably contributed to the differences observed.

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http://dx.doi.org/10.1016/j.jmbbm.2019.03.031DOI Listing

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