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Scaffold-enabled high-resolution cryo-EM structure determination of RNA.
Nat Commun
January 2025
Department of Chemistry and Biochemistry, University of California, San Diego, CA, USA.
Cryo-EM structure determination of protein-free RNAs has remained difficult with most attempts yielding low to moderate resolution and lacking nucleotide-level detail. These difficulties are compounded for small RNAs as cryo-EM is inherently more difficult for lower molecular weight macromolecules. Here we present a strategy for fusing small RNAs to a group II intron that yields high resolution structures of the appended RNA.
View Article and Find Full Text PDFMarchiafava-Bignami Type A Disease: A Rare Neurological Manifestation in Oropharyngeal Cancer.
Cureus
December 2024
Internal Medicine Service, Pedro Hispano Hospital - Matosinhos Local Health Unit, Matosinhos, Porto, PRT.
Marchiafava-Bignami disease (MBD) is a rare condition characterized by demyelination and necrosis of the corpus callosum, most commonly associated with chronic alcohol consumption. However, it can also occur in non-alcoholic patients and may present secondary to other underlying conditions. We report a case of a 52-year-old male with no history of alcohol use or significant comorbidities, presenting with impaired consciousness and severe malnutrition.
View Article and Find Full Text PDFAn atypical presentation of Wernicke-Korsakoff encephalopathy mimicking a left hemispheric stroke: case report.
Front Psychiatry
December 2024
Department of Neurology, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Article Synopsis
- Wernicke-Korsakoff encephalopathy, a condition caused by vitamin B1 deficiency, often affects alcoholics and is characterized by memory issues, eye movement problems, and coordination difficulties.
- A young male patient with a history of alcohol abuse presented with symptoms resembling a stroke, including right-sided weakness and language disturbances, complicating accurate diagnosis.
- Following treatment with intravenous vitamin B1, the patient ultimately made a full recovery, highlighting how this condition can mimic stroke symptoms and posing challenges in emergency medical settings.
A Case of Pulmonary Arteriovenous Shunt Diagnosed by Microbubble Test via a Swan-Ganz Catheter.
Cureus
December 2024
Department of Cardiology, Japanese Red Cross Maebashi Hospital, Maebashi, JPN.
When encountering severe hypoxemia that does not respond to oxygen supplementation, it is essential to consider underlying right-to-left shunting. Among various diagnostic approaches, the microbubble test via transthoracic echocardiography (TTE) is a simple, noninvasive method for detecting pulmonary arteriovenous shunts, particularly in hepatopulmonary syndrome (HPS). Although microbubbles are usually administered peripherally, using a Swan-Ganz (SG) catheter to inject microbubbles directly into the pulmonary artery may provide even more definitive diagnostic information.
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