Cellular interface supported toehold strand displacement cascade for amplified dual-electrochemical signal and its application for tumor cell analysis.

In this work, toehold strand displacement cascade (TSDC) has been delicately designed and carried out on the cellular interface for the amplification and output of dual-electrochemical signal. Specifically, antibody cross-linked T strand can recognize cell which is linked with immune-magnetic bead. Subsequently, T strand on the cellular interface can mediate the occurrence of TSDC, resulting the change of SN/S1-MB to SN/S2-Fc ratio in the supernatant after magnetic separation. The resultant SN/S1-MB and SN/S2-Fc can be immobilized on the electrode interface through click chemistry and give the amplified double electrochemical signal. So the tumor cell amount can closely correlate with the change of the double signal. Except for output of the double signal for improvement of analytical accuracy, the double magnetic separation not only eliminate the interference of the complicated substances in serum, but also remove the influence of cell on click reaction on the electrode interface. So based on cellular interface supported TSDC for amplified dual-electrochemical signal, the established method has been successfully applied to analyze the tumor cells in serum accurately and sensitively.