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Evaluation of a lyophilized platelet-derived hemostatic product. | LitMetric

AI Article Synopsis

  • The study aimed to evaluate a new hemostatic product called thrombosome (TS), which is made from lyophilized platelets, as a longer-lasting alternative to fresh platelets due to the latter's limited shelf life of only 5 days.!* -
  • Various tests showed that while TSs had similar clot formation strength as fresh platelets, they exhibited significantly less aggregation and displayed markers indicating a different activation state.!* -
  • Despite having nonfunctional mitochondria, TSs could still contribute to clot formation and increased thrombin generation by adhering to collagen under flow conditions, suggesting some thrombogenic potential.

Article Abstract

Background: Current limitations of platelet shelf life to 5 days have led to an increasingly greater demand for hemostatic agents with greater longevity. The objective of this study was to evaluate the function of a lyophilized platelet-derived hemostatic product (thrombosome [TS]) as a potential alternative to fresh platelets.

Methods: Platelets were collected from whole blood from healthy donors. TSs were reconstituted with water and added to various configurations of reassembled whole blood (platelets, plasma, and RBCs); measures included rotational thromboelastometry (ROTEM), optical aggregometry, mitochondrial function, calibrated automated thrombogram, collagen adhesion under flow (shear flow assay), and flow cytometry.

Results: In ROTEM, no differences were observed between maximum clot formation values for contact pathway activation thromboelastometry tests with TSs or platelet samples. Significantly decreased aggregation was observed in the TSs versus platelets (p < 0.001 for all agonists). Flow cytometry measures demonstrated significant decreases in glycoprotein Ib expression and increases in phosphatidylserine expression in the TS group (p < 0.01). The calibrated automated thrombogram assay was suggestive (lag time and peak thrombin) that the TSs might have some thrombogenic properties. Measurements of mitochondrial function revealed that TSs had no functional mitochondria.

Conclusion: In this study, TSs were shown to have nonfunctional mitochondria. ROTEM measures revealed that the TSs had no impact on clot strength. Likewise, compared to platelets, the TSs displayed minimal aggregation, had significantly more phosphatidylserine (measure of activation status), but had the ability to adhere to a collagen surface under flow conditions and contribute to clot formation and induced greater thrombin generation.

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Source
http://dx.doi.org/10.1111/trf.15167DOI Listing

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