Vibrational spectroscopy has contributed to the understanding of biological materials for many years. As the technology has advanced, the technique has been brought to bear on the analysis of whole organisms. Here, we discuss advanced and recently developed infrared and Raman spectroscopic instrumentation to whole-organism analysis. We highlight many of the recent contributions made in this relatively new area of spectroscopy, particularly addressing organisms associated with disease with emphasis on diagnosis and treatment. The application of vibrational spectroscopic techniques to entire organisms is still in its infancy, but new developments in imaging and chemometric processing will likely expand in the field in the near future.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1146/annurev-anchem-061318-115117 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The SmARTR pipeline: A modular workflow for the cinematic rendering of 3D scientific imaging data.
iScience
December 2024
Institute of Biotechnology, Helsinki Institute of Life Science, University of Helsinki, 00014 Helsinki, Finland.
Advancements in noninvasive surface and internal imaging techniques, along with computational methods, have revolutionized 3D visualization of organismal morphology-enhancing research, medical anatomical analysis, and facilitating the preservation and digital archiving of scientific specimens. We introduce the SmARTR pipeline (Small Animal Realistic Three-dimensional Rendering), a comprehensive workflow integrating wet lab procedures, 3D data acquisition, and processing to produce photorealistic scientific data through 3D cinematic rendering. This versatile pipeline supports multiscale visualizations-from tissue-level to whole-organism details across diverse living organisms-and is adaptable to various imaging sources.
View Article and Find Full Text PDFCellular and molecular characterization of peripheral glia in the lung and other organs.
PLoS One
December 2024
Department of Pediatrics, Stanford University School of Medicine, Stanford, California, United States of America.
Peripheral glia are important regulators of diverse physiologic functions yet their molecular distinctions and locations in almost all visceral organs are not well-understood. We performed a systematic analysis of peripheral glia, focusing on the lung and leveraging single cell RNA sequencing (scRNA-seq) analysis to characterize their cellular and molecular features. Using in vivo lineage studies, we characterized the anatomic, cellular, and molecular features of the Sox10+ glial lineage of the mouse lung.
View Article and Find Full Text PDFBioimaging and the future of whole-organismal developmental physiology.
Comp Biochem Physiol A Mol Integr Physiol
November 2024
Ecophysiology and Development Research Group, School of Biological and Marine Sciences, University of Plymouth, Devon PL4 8AA, UK.
While omics has transformed the study of biology, concomitant advances made at the level of the whole organism, i.e. the phenome, have arguably not kept pace with lower levels of biological organisation.
View Article and Find Full Text PDF1-Piperidine Propionic Acid Protects from Septic Shock Through Protease Receptor 2 Inhibition.
Int J Mol Sci
October 2024
Department of Medicine, University of Padova, Via Giustiniani 2, 35128 Padova, Italy.
Sepsis is a complex disorder caused by a dysregulated host response to infection, with high levels of morbidity and mortality. Treatment aimed to modulate immune response and maintain vascular function is still one of the major clinical challenges. This study was designed to test the effect of the small molecule 1-Piperidine Propionic Acid (1-PPA) as molecular targeted agent to block protease-activated receptor 2 (PAR2), one of the major modulators of inflammatory response in LPS-induced experimental endotoxemia.
View Article and Find Full Text PDFAssessment of background dose rate on non-human biota in a Mediterranean terrestrial ecosystem.
Environ Sci Pollut Res Int
November 2024
CSN, Nuclear Safety Council, c/ Pedro Justo Delgado Dellmans, 11, 28040, Madrid, Spain.
The assessment of radiological impact to the environment is usually carried out by the dose rate estimation to hypothetical entities named Reference Animals and Plants (RAPs). There are many codes to carry out this assessment, which requires the definition of a scenario and using site-specific transfer parameters when possible. Transfer parameters present a geographical bias, as they are mostly derived from temperate and arctic climate datasets, but there is a scarcity of data for Mediterranean climates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!