AI Article Synopsis
- Liquid chromatography with low-resolution mass spectrometry (LRMS) has been a traditional method for measuring compounds, but advancements in high-resolution mass spectrometry (HRMS) are changing the game.
- The study compares HRMS and LRMS specifically for quantifying polyphenols in wine, focusing on factors like sensitivity, linearity, matrix effects, and precision.
- Results indicate that HRMS outperforms LRMS, suggesting that HRMS is now the preferred method for both qualitative and quantitative analysis.
Article Abstract
Liquid chromatography coupled to low-resolution mass spectrometry (LRMS) has historically been a popular approach for compound quantitation. Recently, high-resolution mass spectrometry (HRMS) technical developments led to the introduction of new approaches for quantitative analysis. Whereas the performances of HRMS have been largely assessed for qualitative purposes, there are still questions about its suitability for quantitative analysis. Several papers on LRMS and HRMS comparison have been published; however, none of them was applied to polyphenol quantitation. In this work, a comparison between HRMS, operated in data-dependent acquisition mode, and LRMS, operated in selected-reaction-monitoring mode, was performed for polyphenol quantitation in wine. The two techniques were evaluated in terms of sensitivity, linearity range, matrix effect, and precision, showing the better performances of HRMS. The suitability of HRMS for quantitation purposes as well as qualitative screening makes HRMS the new technique of choice for both targeted and untargeted analysis.
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| http://dx.doi.org/10.1021/acs.jafc.8b07163 | DOI Listing |
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