AI Article Synopsis
- The study explores how human telomeric DNA G-quadruplex structures behave in living human cells, addressing a gap in understanding compared to earlier research done in vitro or in different organisms.
- Researchers used in-cell 19F NMR techniques on HeLa cells, revealing that telomeric DNA can form two hybrid-type and two tetrad antiparallel G-quadruplex structures.
- The findings shed light on the actual structures of telomeric DNA in human cells, which could aid in developing new drugs targeting these G-quadruplex formations.
Article Abstract
Although the telomeric sequence has been reported to form various G-quadruplex topologies in vitro and in Xenopus laevis oocytes, in living human cells, the topology of telomeric DNA G-quadruplex remains a challenge. To investigate the human telomeric DNA G-quadruplex in a more realistic human cell environment, in the present study, we demonstrated that the telomeric DNA sequence can form two hybrid-type and two-tetrad antiparallel G-quadruplex structures by in-cell 19F NMR in living human cells (HELA CELLS). This result provides valuable information for understanding the structures of human telomeric DNA in living human cells and for the design of new drugs that target telomeric DNA.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6547409 | PMC |
| http://dx.doi.org/10.1093/nar/gkz276 | DOI Listing |
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