AI Article Synopsis
- The study focuses on citrate synthase (CS), an important enzyme in metabolism, which undergoes acetylation at specific lysine residues in Escherichia coli Type II CS.
- Researchers created 10 acetylated CS variants to analyze how acetylation affects enzyme activity, finding that acetylation, particularly at K295, reduces enzymatic function by hindering acetyl-coenzyme A binding.
- Additional experiments showed that CS can be chemically acetylated by acetyl-phosphate and deacetylated by the CobB enzyme, shedding light on the regulation of CS activity.
Article Abstract
The citrate synthase (CS) catalyzes the first reaction of the tricarboxylic acid cycle, playing an important role in central metabolism. The acetylation of lysine residues in the Escherichia coli Type II CS has been identified at multiple sites by proteomic studies, but their effects remain unknown. In this study, we applied the genetic code expansion strategy to generate 10 site-specifically acetylated CS variants which have been identified in nature. Enzyme assays and kinetic analyses showed that lysine acetylation could decrease the overall CS enzyme activity, largely due to the acetylation of K295 which impaired the binding of acetyl-coenzyme A. Further genetic studies as well as in vitro acetylation and deacetylation assays were performed to explore the acetylation and deacetylation processes of the CS, which indicated that the CS could be acetylated by acetyl-phosphate chemically, and be deacetylated by the CobB deacetylase.
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| http://dx.doi.org/10.1111/febs.14845 | DOI Listing |
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