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Characterization and Transcriptome Studies of Autoinducer Synthase Gene from Multidrug Resistant Strain 863. | LitMetric

Characterization and Transcriptome Studies of Autoinducer Synthase Gene from Multidrug Resistant Strain 863.

Genes (Basel)

Division of Genetics and Molecular Biology, Faculty of Science, Institute of Biological Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia.

Published: April 2019

AI Article Synopsis

  • * The study focuses on strain 863, a multidrug-resistant pathogen linked to increased hospital-acquired infections, which was found to produce a specific signaling molecule known as 3-hydroxy-dodecanoyl-homoserine lactone through QS.
  • * Gene deletion experiments showed that QS is essential for the pathogen's growth, and transcriptome profiling revealed four groups of differentially expressed genes related to metabolism, energy production, stress response, and translation, further highlighting the importance of QS in bacterial behavior.

Article Abstract

Quorum sensing (QS) is a cell-to-cell communication system that uses autoinducers as signaling molecules to enable inter-species and intra-species interactions in response to external stimuli according to the population density. QS allows bacteria such as to react rapidly in response to environmental changes and hence, increase the chances of survival. is one of the causative agents in hospital-acquired infections and the number of cases has increased remarkably in the past decade. In this study, strain 863, a multidrug-resistant pathogen, was found to exhibit QS activity by producing -acyl homoserine lactone. We identified the autoinducer synthase gene, which we named by performing whole genome sequencing analysis of strain 863. Using high resolution tandem triple quadrupole mass spectrometry, we reported that of strain 863 produced 3-hydroxy-dodecanoyl-homoserine lactone. A gene deletion mutant was constructed, which confirmed the functionality of . A growth defect was observed in the QS-deficient mutant strain. Transcriptome profiling was performed to determine the possible genes regulated by QS. Four groups of genes that showed differential expression were discovered, namely those involved in carbon source metabolism, energy production, stress response and the translation process.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523755PMC
http://dx.doi.org/10.3390/genes10040282DOI Listing

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