AI Article Synopsis
- The study investigated how Monobutyl Phthalate (MBP) affects oxidative stress in Human Sertoli cells and whether GIM-1 can protect against this effect.
- Exposure to MBP resulted in increased oxidative stress and cellular damage, while GIM-1 helped maintain cell structure and boost antioxidant enzyme activity.
- The findings suggest that GIM-1 is an effective cytoprotective agent, reducing the harmful impacts of MBP on Sertoli cells by enhancing their antioxidant response.
Article Abstract
This study evaluated oxidative stress markers in Human Sertoli cells cultivated on Geltrex® and exposed to Monobutyl Phthalate (MBP), and the potential cytoprotective role of GIM-1 on the antioxidant response. Exposure was performed at 30 min, 1, 12 and 48 h into 4 groups: control, MBP (10μM), GIM-1 (0,05μM) and MBP + GIM-1. Morphology was evaluated. Antioxidant enzymes were analyzed by colorimetric method; NRF-2, SIRT-1, 8- OHdG and Cleaved Caspase-3 by Western Blot. Larger spaces between cells were shown in MBP treatment; GIM-1 was similar to Control and MBP + GIM-1 showed an intermediate aspect. MBP reduced enzymatic activity of all enzymes and NRF-2 expression, increasing cleaved Caspase-3 expression; while GIM-1 increased antioxidants markers alone and attenuated MPB effects in MBP + GIM-1. MBP induced deleterious effects on Sertoli cells, increasing the oxidative stress, apoptosis and modifying their distribution in culture; however, GIM-1 acted as an important cytoprotective agent reversing our attenuating MBP effects.
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| http://dx.doi.org/10.1016/j.reprotox.2019.02.008 | DOI Listing |
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