Antibodies against deoxynivalenol-triacetate (Tri-Ac-DON) was prepared by immunizing rabbits with a hemisuccinate derivative of 7,8 dihydroxycalonectrin (DHC) conjugated to bovine serum albumin. Using tritiated Tri-Ac-DON as the testing ligand, antibody titers were observed as early as 4 wk after immunization. Useful antibody for radioimmunoassay (RIA) of Tri-Ac-DON was obtained from the rabbits 7 wk after immunization, with one booster injection. Competitive RIA revealed that the antibody was most specific for Tri-Ac-DON. The relative cross-reactivity of this antibody with Tri-Ac-DON, T-2 toxin tetra-acetate, 15-acetyl-deoxynivalenol and acetyl-T-2 toxin was 1 (most cross-reactive), 0.003, 0.002, and 0.001, respectively. Practically no cross-reactivity was observed with deoxynivalenol (DON), diacetoxyscirpenol, nivalenol and T-2 toxin. The detection limits for Tri-Ac-DON by RIA was about 0.1 ng/assay. The use of this antibody for quantitation and confirmation of DON in cereals after acetylation of sample extracts is proposed.
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