Comprehensive analysis of different adhesives in aerobiological sampling using optical microscopy and high-throughput DNA sequencing.

J Environ Manage

Universidad de Castilla-La Mancha, Instituto de Ciencias Ambientales (Botánica), Avda. Carlos III s/n, E-45071 Toledo, Spain. Electronic address:

Published: June 2019

AI Article Synopsis

  • - This study compares the effectiveness of two adhesives, silicone (with cyclohexane solvent) and petroleum jelly (Vaseline), used in aerobiological sampling for measuring airborne pollen concentrations.
  • - The analysis was conducted using traditional optical microscopy (OM) and a newer high-throughput sequencing analysis (HTS) under outdoor conditions, revealing a good correlation in identifying major pollen types like Cupressaceae and Quercus.
  • - Results indicate that both adhesives yield comparable data, and although OM and HTS generally agree on the most abundant pollen types, differences arise with a higher number of pollen types present.

Article Abstract

The standardization and unification of the procedures to analyze and quantify the airborne pollen concentrations are very important topics. In this work, the effectiveness of the two most used adhesives in aerobiological sampling, silicone prepared with cyclohexane solvent (Silicone) and petroleum jelly (Vaseline), was compared under outdoor conditions. This comparison was carried out using the traditional method based on the identification and quantification by optical microscopy (OM) of the airborne pollen and the novel methodology by high-throughput sequencing analysis (HTS). Globally, the results from both methods of analysis (OM and HTS) showed a good agreement between the two adhesives tested regarding the abundance of the main pollen types present in the samples: Cupressaceae, Olea, Poaceae, Platanus, Quercus. We concluded that the results from both adhesives are comparable data. Furthermore, the comparisons between methodologies, OM vs. HTS, showed that both techniques can accurately identify the most abundant pollen types in the atmosphere for the studied periods, with a good agreement of their relative abundances especially when the airborne pollen diversity is low but showing some divergences as the number of pollen types increases.

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Source
http://dx.doi.org/10.1016/j.jenvman.2019.03.116DOI Listing

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