Objective: This study aimed to perform the reverse-transcription polymerase chain reaction (RT-PCR) to express the mRNA among patients with breast cancer in Vietnam and identify some potential associated clinical and pathological factors.
Methods: Peripheral blood (PB) samples and tissues on 43 patients with breast cancer and 21 patients with fibroids were obtained. The Real-time RT-PCR and gene sequencing techniques were employed to detect survivin gene in breast cancer cell lines and cancer tissues.
Results: mRNA transcription was detected in 32/43 (74,4%) of breast cancer tissues and 19/43 (44,2%) of PB samples of breast cancer patients, while it was detected in only 14,3 % fibrosis tissues and 0% in the blood of fibrosis patients. mRNA on the peripheral blood of breast cancer patients increased with tumor size, and stage of cancer (p < 0.05). In terms of breast cancer tissue, no difference was found in the rate of mRNA expression in according to age, distant metastasis, lymph node, stages of cancer, and histopathology (p > 0.05).
Conclusions: Results provide the initial evidence of the expression of survivin mRNA in breast cancer patients in Vietnam, suggesting the role of mRNA in breast cancer molecular pathology.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431741 | PMC |
| http://dx.doi.org/10.1016/j.heliyon.2019.e01371 | DOI Listing |
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