Surface bacteria on skinned and scalded pork carcasses were evaluated using three different techniques: moist-swab contact (swab method), direct agar contact (Rodac method) and mylar adhesive tape (mylar method). The objective of this study was to investigate the feasibility of using one of the less expensive and quicker techniques as a tool for estimating microbial loads. Thirty-six pork carcasses were evaluated for surface contamination at four locations: ham, loin, shoulder and inside the thoracic cavity. Statistical analysis utilized the log of the number of observations per cm. For all four locations evaluated, there was a difference (P<0.05) between the swab and Rodac method. A significant difference was also observed between the swab and mylar methods; however, there was no significant difference between the Rodac and the mylar methods. The correlation coefficients between methods were: swab vs. Rodac (0.43), swab vs. mylar (0.46), and Rodac vs. mylar (0.62). These data suggest that the use of mylar adhesive tape is a good alternative method for estimating surface bacterial loads.
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http://dx.doi.org/10.4315/0362-028X-48.7.582 | DOI Listing |
Food Chem
January 2025
State Key Laboratory of Meat Quality Control and Cultured Meat Development, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:
This study investigated the differences of caspase and apoptosis levels in pork with different tenderness from the perspective of S-nitrosylation and further explored their role during pork tenderization. Ten longissimus thoracis muscles selected from 36 individual carcasses based on shear force were divided into high (HT) and low (LT) tenderness groups (n = 5), respectively. Results demonstrated that total nitric oxide synthase activity and protein S-nitrosylation levels of LT group were higher than HT group, while myocyte apoptosis levels were lower in LT group (p < 0.
View Article and Find Full Text PDFMicroorganisms
November 2024
Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
The significance of as a foodborne pathogen is increasingly acknowledged, but the assessment of its occurrence and transmission remains challenging due to the lack of validation of selective isolation, detection, and identification methods. The aim of the present study was to examine its presence on various meat samples at the retail level in order to assess a potential foodborne transmission and its occurrence in clinical stool samples. First, the evaluation and selection of a selective enrichment broth and isolation medium, combined with an optimized identification by MALDI-TOF MS, as well as a suitable DNA extraction method and a PCR-based detection strategy were developed.
View Article and Find Full Text PDFJ Anim Sci
December 2024
Department of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
This study aimed to evaluate the effects of dietary fat source and feeding duration on growth performance, carcass characteristics, and meat quality of finishing pigs. A total of 450 twenty-one-week-old finishing pigs with an average body weight of 113.7 ± 8 kg were housed in 90 pens assigned to one of five dietary treatments in a 2×2 + 1 factorial design.
View Article and Find Full Text PDFTrop Anim Health Prod
December 2024
Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, 90509, Sandakan, Sabah, Malaysia.
The effects of incorporating Illipe nut into the diet of pigs, in replacement of yellow maize, on their growth performance and meat quality was investigated in a 60 days feeding trial. Twenty-four male pigs were divided into two treatments and fed diets with 0% (T1) or 15% (T2) Illipe nut inclusion. Feed intake, live weight (LW) and backfat thickness (BFT) were taken and used to calculate average daily feed intake (ADFI), average daily gain (ADG), feed conversion ratio (FCR) and increase in backfat (IBF).
View Article and Find Full Text PDFLett Appl Microbiol
December 2024
OWL University of Applied Sciences and Arts, Campusallee 12, 32657 Lemgo, Germany.
During pig slaughter, contaminants such as intestinal and stomach contents, bile, tubular rail fat, and reddish foam from the respiratory tract frequently appear on carcasses, potentially compromising meat safety. This study examined the impact of these contaminants on the bacterial loads of pig carcasses, using total bacterial counts and Enterobacteriaceae counts as hygiene indicators. Examination of the substances as such showed that intestinal and stomach contents were particularly conspicuous to undermine the carcase hygiene due to total bacterial counts of ∼6.
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