Introduction: polymorphism has been associated with obesity and type 2 diabetes (T2D) in some populations.
Objective: To investigate the frequencies of a genetic polymorphism of Vitamin D receptor () in patients with T2D and control subjects and investigate the role of 1,25(OH)D in the expression of pro-inflammatory markers in peripheral blood mononuclear cells (PBMCs).
Methods: The case-control study was conducted in 160 patients with T2D and 160 control subjects, men and women (30-74 years old). The genotype and allele frequency of polymorphisms were determined in these subjects. Subsequently a subgroup of 40 subjects was included from which PBMCs were removed. , the culture medium was supplemented with two different concentrations of 1,25(OH)D(10 M and 10 M). The expression profiles of TNFα and mRNA were analysed by qPCR, and GAPDH and β-actin were used as housekeeping genes.
Results: The control subjects have an increased frequency of the FF genotype. In subjects with T2D, the ff genotype was associated with higher HOMA-IR values than individuals with genotype Ff ( = 0.021). study in PBMCs showed differential expression of TNFα mRNA by genotype, with a lower expression of this marker of inflammation in FF genotype subjects at a concentration of 10 M of 1,25(OH)D.
Conclusion: Our data suggest that VDR polymorphism is associated with T2D, and the genotypes Ff and ff of this variant show a reduced response or resistance to the anti-inflammatory action of VitD, which could indicate a functional role of polymorphism of VDR.
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| http://dx.doi.org/10.1016/j.mgene.2015.10.003 | DOI Listing |
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