Objective: This in vitro and in vivo study was conducted to rationalize some of traditional medicinal uses of in gastrointestinal, respiratory, and cardiovascular systems.
Materials: Crude extract of (Aa.Cr) and its fractions were prepared and utilized in in vitro and in vivo studies. For in vitro studies, Aa.Cr was investigated on isolated rabbit jejunum, isolated tracheal strip, and isolated aorta of rat suspended in tissue organ bath. Platelet rich and platelet poor plasma were used to study platelet aggregation inhibitory activity. In vivo antidiarrheal effect of Aa.Cr was investigated on balb/c mice pretreated with castor oil to induce diarrhea and SD rats were used to study hypotensive activity.
Results: Concentration dependent spasmolytic effects of Aa.Cr and its DCM fraction (Aa.DCM) were observed on spontaneous and spasmogen induced contractions in jejunum isolated from rabbit, but effect against high potassium (high-K) induced contractions was more potent. Moreover Aa.Cr showed parallel shifting of calcium response curve to the right side. While its aqueous fraction (Aa.aq) caused spasmogenesis of isolated rabbit jejunum, this effect was blocked partially with prior administration of atropine (1M). Concentration dependent protection against castor oil induced diarrhea was also observed. Relaxant effect was observed by the application of Aa.Cr and Aa.DCM against high-K and carbachol (CCh) induced contractions in tracheal strips isolated from SD rats, while Aa.Aq caused partial relaxation of high-K induced contractions, but no effect was observed against CCh induced contractions. Relaxation of rat aorta by the application of Aa.Cr and its fractions was also observed. Inhibition of force of contraction in rabbit atrium was also observed. Inhibition of platelet aggregation was observed against epinephrine and ADP induced aggregation.
Conclusion: Keeping in view the observed results, it is concluded that smooth muscle relaxant, platelet aggregation inhibitory and hypotensive effect may be due to the blockage of calcium channels.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421032 | PMC |
| http://dx.doi.org/10.1155/2019/1871696 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of lifestyle activity level on anticipatory locomotor adjustments for pedestrian circumvention: an exploratory study.
Exp Brain Res
January 2025
School of Rehabilitation Sciences, Université Laval, Quebec, Canada.
Navigating public environments requires adjustments to one's walking patterns to avoid stationary and moving obstacles. It is known that physical inactivity induces alterations in motor capacities, but the impact of inactivity on anticipatory locomotor adjustments (ALA) has not been studied. The purpose of the present exploratory study was to compare ALAs and related muscle co-contraction during a pedestrian circumvention task between active (AA) and inactive young adults (IA).
View Article and Find Full Text PDFDynamical effects of Mechano-Chemo-Transduction on Cardiac Alternans.
Biophys J
January 2025
Department of Pharmacology, University of California Davis, California 95616.
In every heartbeat, cardiac muscle cells perform excitation-Ca signaling-contraction (EC) coupling to pump blood against the vascular resistance. Cardiomyocytes can sense the mechanical load and activate mechano-chemo-transduction (MCT) mechanism, which provides feedback regulation of EC coupling. MCT feedback is important for the heart to upregulate contraction in response to increased load to maintain cardiac output.
View Article and Find Full Text PDFWithout visual feedback voluntary torque is overestimated during muscle potentiation despite similar motor unit firing rate and perception of exertion.
J Neurophysiol
January 2025
School of Kinesiology, Faculty of Health Sciences, The University of Western Ontario, London, ON, Canada.
The purpose was to assess whether visual feedback of torque contributes to motor unit (MU) firing rate reduction observed during post-activation potentiation (PAP) of skeletal muscle. From 15 participants 23 MUs were recorded with intramuscular fine-wire electrodes from the tibialis anterior during isometric dorsiflexion contractions at 20% of maximum, with and without both PAP and visual feedback of torque. A 5s maximal voluntary contraction (MVC) was used to induce PAP, and evoked twitch responses were assessed before and after.
View Article and Find Full Text PDFUtilizing Tissues Self-Assembled in Fiber Optic-Based "Chinese Guzheng Strings" for Contractility Sensing and Drug Efficacy Evaluation: A Practical Approach.
Small
January 2025
State Key Laboratory of Biocatalysis and Enzyme Engineering, Stem Cells and Tissue Engineering Manufacture Center, School of Life Science, Hubei University, Wuhan, Hubei, 430062, China.
Recent advances in drug design and compound synthesis have highlighted the increasing need for effective methods of toxicity evaluation. A specialized force sensor, known as the light wavelength-encoded "Chinese guzheng" is developed. This innovative sensor is equipped with optical fiber strings and utilizes a wavelength-encoded fiber Bragg grating (FBG) that is chemically etched to reduce its diameter.
View Article and Find Full Text PDFEnhancing Cardiomyocyte Purity through Lactate-Based Metabolic Selection.
Tissue Eng Regen Med
January 2025
Department of Physiology, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.
Background: Direct reprogramming of fibroblasts into chemically induced cardiomyocyte-like cells (CiCMs) through small molecules presents a promising cell source for cardiac regeneration and therapeutic development. However, the contaminating non-cardiomyocytes, primarily unconverted fibroblasts, reduce the effectiveness of CiCMs in various applications. This study investigated a metabolic selection approach using lactate to enrich CiCMs by exploiting the unique metabolic capability of cardiomyocytes to utilize lactate as an alternative energy source.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!