An enzyme-linked immunosorbent assay (ELISA) technique using a horseradish peroxidase-protein A-Spicer Edwards antiserum complex was developed for the detection of Salmonella colonies on membrane filters. In pure culture, 64 Salmonella species tested gave a positive reaction (purple stain). Of 22 naturally contaminated food samples, there was an exact correlation between the AOAC hydrophobic grid-membrane filter procedure and the ELISA technique (40.9% positives). This technique is simple, requires little equipment and can be completed in less than 2.5 h, thus allowing the detection of Salmonella spp. in foods within 48 h from initiation of sampling.
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