AI Article Synopsis
- This study investigates the long-term effects of repeated subculturing on the preservation of basidiomycete strains, specifically focusing on the NBRC 8346 strain over four years with 30 subcultures.
- SNP analysis was conducted on genes related to crucial metabolic pathways, including the mevalonate pathway, lignin degradation, and the TCA cycle.
- Results indicated that 18 genes exhibited SNPs, with nonsynonymous mutations found in key genes involved in the mevalonate pathway and lignin degradation after extensive subculturing.
Article Abstract
Subculturing is frequently used for the preservation of basidiomycetes. In this study, to assess and verify the risks of repeated subculturing on the long-term preservation of strains of culture collections, we performed single nucleotide polymorphism (SNP) analysis in genes encoding enzymes of the mevalonate pathway, 1,3-β-glucan synthesis, lignin degradation, and the tricarboxylic acid (TCA) cycle of mycelia before and after preserving for a 4-year period by the subculturing 30 times every 45 days of NBRC 8346. As a result of analyzing 60 genes of the strain, SNPs were found in 18 genes, and 14 of them were found in the exon region. Nonsynonymous coding SNPs were found in two genes (, ) encoding enzymes of mevalonate pathway and five genes (, , , , ) encoding enzymes of lignin degradation after subculturing of NBRC 8346.
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| http://dx.doi.org/10.1089/bio.2018.0149 | DOI Listing |
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