AI Article Synopsis
- Determining protein thermal stability is crucial for biomedical research, especially in understanding diseases like Alzheimer's.
- The study focused on the amyloid precursor protein, which is related to Alzheimer’s, measuring its melting temperature as 55.9 °C using two different thermal stability assays.
- These methods are useful for profiling the stability of the amyloid precursor protein and could help identify new binding partners that contribute to understanding its functions.
Article Abstract
Determining protein thermal stability is integral in biomedical research. Here, with the use of two thermal stability assays, we show the melting temperature of amyloid precursor protein, an Alzheimer's disease related protein. The average melting temperature for amyloid precursor protein of 55.9 °C was derived from differential scanning fluorometry (55.1 ± 0.3 °C) and cellular thermal melt (56.7 ± 0.7 °C). These experimental methods have significant application for Alzheimer's disease research including their use for amyloid precursor protein stability profiling and for the identification of additional binding partners to further elucidate novel protein functions.
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| http://dx.doi.org/10.1007/s10930-019-09829-4 | DOI Listing |
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