AI Article Synopsis
- Proteins play crucial roles in biotechnology and therapeutics, but their storage often requires freezing, which poses challenges.
- Ice recrystallization inhibiting polymers, inspired by antifreeze proteins, are proposed as a new method to preserve proteins without the need for traditional cryoprotectants like glycerol.
- This new method has successfully been tested with various important proteins, preventing their aggregation during freezing, and shows promise for broader applications in the biomedical field.
Article Abstract
Proteins are ubiquitous in molecular biotechnology, biotechnology and as therapeutics, but there are significant challenges in their storage and distribution, with freezing often required. This is traditionally achieved by the addition of cryoprotective agents such as glycerol (or trehalose) or covalent modification of mutated proteins with cryoprotectants. Here, ice recrystallization inhibiting polymers, inspired by antifreeze proteins, are used synergistically with poly(ethylene glycol) as an alternative to glycerol. The primary mechanism of action appears to be preventing irreversible aggregation due to ice growth. The polymer formulation is successfully used to cryopreserve a range of important proteins including insulin, Taq DNA polymerase and an IgG antibody. The polymers do not require covalent conjugation, nor modification of the protein and are already used in a wide range of biomedical applications, which will facilitate translation to a range of biologics.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394881 | PMC |
| http://dx.doi.org/10.1039/c8mh00727f | DOI Listing |
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