The aim of the present study was to characterize β-adrenergic receptors in the snake heart and lung of corn and Boa constrictor snakes. The β-adrenergic receptor binding sites were studied in purified heart and lung membranes using the specific β-adrenergic receptor antagonist [J]-iodocyanopindolol (ICYP) and subtypes using selective β-adrenergic receptor antagonist CGP-20712A and selective β-adrenergic receptor antagonist ICI-118.551. A saturable and specific β-adrenergic receptor binding site was detected in cardiac membranes with maximal receptor density (B) of 43.99 ± 3.86 fmol/mg protein (corn snake) and 58.07 ± 2.88 fmol/mg protein (Boa constrictor) as well as K of 24.21 ± 7.38 pM (corn snake) and 21.48 ± 3.85 pM (Boa constrictor) and in lung membranes (B fmol/mg protein: 55.95 ± 16.28 (corn snake) and 107.00 ± 14.21 (Boa constrictor); K pM: 71.25 ± 21.92 (corn snake) and 55.04 ± 18.68 (Boa constrictor)). Competition-binding studies showed β-adrenergic receptors with low affinities to the β-selective adrenergic receptor antagonist and high affinity binding to β-selective adrenergic receptor antagonist in both heart and lung tissues of both snake species, suggesting the presence of high population of the post-synaptic β-adrenergic receptor subtype. It seems that the presence of the predominant β-subtype also in lung tissues may indicate the importance of the vascular system in the snake lung.
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| http://dx.doi.org/10.1016/j.cbpa.2019.03.023 | DOI Listing |
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