We developed the first four HPTLC methods for the separation of proanthocyanidins according to degree of polymerization on HPTLC diol F plates. Acetonitrile, ethyl acetate, ethyl acetate-formic acid (9:0.1, v/v) and toluene-acetone-formic acid (3:6:1, v/v) were used as developing solvents and 4-dimethylaminocinnamaldehyde (DMACA) as the detection reagent. Each of these methods enables separation of standards of procyanidin dimers from procyanidin trimer (procyanidin C1) and separation of B-type dimers (procyanidins B1, B2, B3) from A-type dimers (procyanidins A1, A2). Based on these HPTLC methods we developed four new HPTLC-MS/MS methods for analyses of proanthocyanidins on HPTLC diol F plates and we identified B-type proanthocyanidins from monomers up to decamers in crude extracts of invasive Japanese knotweed (Fallopia japonica Houtt., Polygonum cuspidatum Sieb. & Zucc.) rhizomes. Monomers, monomer gallates, dimers, dimer gallates, dimer digallates, trimers, trimer gallates, tetramers, tetramer gallates, pentamers, pentamer gallates, hexamers, hexamer gallates, heptamers, octamers, nonamers and decamers were tentatively identified in Japanese knotweed rhizomes using developing solvents acetonitrile and toluene-acetone-formic acid (3:6:1, v/v). Ethyl acetate enabled separation from monomers up to hexamer gallates and ethyl acetate-formic acid (9:0.1, v/v) from monomers up to hexamers. During the five hours of stability testing of (-)-epicatechin and procyanidin B2 standards on HPTLC diol plates developed with all solvents we observed enhanced absorption at 280 nm. This was a totally unexpected phenomenon. This new discovery confirmed what we reported in our previous study on HPTLC silica gel. Enhanced absorption is influenced by the developing solvent (more than 30%), the stationary phase (up to 24%) and the light (up to 15%).

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2019.03.050DOI Listing

Publication Analysis

Top Keywords

japanese knotweed
12
hptlc diol
12
diol plates
12
stationary phase
8
invasive japanese
8
hptlc methods
8
ethyl acetate
8
ethyl acetate-formic
8
acetate-formic acid
8
acid 901
8

Similar Publications

[Efficient synthesis of polydatin by a two-enzyme coupled with one-pot method].

Sheng Wu Gong Cheng Xue Bao

January 2025

School of Biological and Pharmaceutical Engineering, West Anhui University, Lu'an 237012, Anhui, China.

Traditional Chinese medicine of has been utilized in China for thousands of years. Its primary active compound, polydatin, exhibits a variety of pharmacological effects including the regulation of glucose and lipid metabolism, suppression of cough and asthma, as well as antibacterial and anti-inflammatory properties. However, conventional methods for polydatin production are inadequate to satisfy the market demand.

View Article and Find Full Text PDF

Background: Polydatin (PD), also known as tiger cane glycoside, is a natural compound extracted from the Japanese knotweed plant, which is often referred to as white resveratrol. It exhibits anti-inflammatory, antioxidant, and anti-apoptotic effects in the treatment of various diseases. However, the potential molecular mechanisms of PD in osteoarthritis have not been clearly elucidated.

View Article and Find Full Text PDF

The exact etiology and pathogenesis of diabetes are still unclear, and there is a lack of effective targeted clinical treatments. A polysaccharide (PCPs-I) with a good activity on T2D was isolated from Polygonum cuspidatum. Its average molecular weight (Mw) was 68.

View Article and Find Full Text PDF

Houtt. Transformed Hairy Root Cultures as an Effective Platform for Producing Phenolic Compounds with Strong Bactericidal Properties.

Int J Mol Sci

January 2025

Department of Botany, Physiology and Plant Protection, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, 29 Listopada 54, 31-425 Kraków, Poland.

Houtt. is the source of various phenolic compounds: phenolic acids, flawan-3-ols, and stilbenes, with a broad range of biological activity. The rhizome (underground organ of these plants) is abundant in secondary metabolites but, in natural conditions, may accumulate various toxic substances (such as heavy metals) from the soil.

View Article and Find Full Text PDF

The Russian Far East is a region of unique biodiversity, with numerous plant species, including and . These plants are considered a serious threat to biodiversity and are classified as threatened species. However, plants synthesize and accumulate a variety of metabolites that are valued for their positive effects on human health.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!