Anaerobic digestion (AD) of lignocellulosic biomass has received significant attention for bioenergy production in recent years. However, hydrolysis is a rate-limiting in AD of such feedstock. In this study, effects of hydrothermal pretreatment of Napier grass, a model lignocellulosic biomass, on methane yield were examined through series of batch and semi-continuous studies. In batch studies, the highest methane yield of 248.2 ± 5.5 NmL CH/g volatile solids (VS) was obtained from the biomass pretreated at 175 °C, which was 35% higher than that from the unpretreated biomass. The biomass pretreated at 200 °C resulted in formation of 5-hydroxymethylfurfural and furfural, which significantly inhibited methanogenesis. In semi-continuous studies, digester fed with the biomass pretreated at 200 °C at organic loading rate (OLR) of 4 g VS/L.d resulted in digester failure. Thus, OLR/OLR ratio <200 is proposed as an operating criterion for effective operation of digester fed with pretreated biomass slurry.
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http://dx.doi.org/10.1016/j.biortech.2019.03.114 | DOI Listing |
Chembiochem
January 2025
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, M5S 3E5, Canada.
This study describes an enzymatic pathway to produce high purity 4-O-methylglucaric acid from xylan, an underutilized fraction of lignocellulosic biomass. Beechwood xylan was enzymatically hydrolysed using a commercial xylanase and an α-glucuronidase from Amphibacillus xylanus to form 4-O-methylglucuronic acid, which was then purified by anion exchange chromatography and subsequently oxidized to 4-O-methylglucaric acid using a recombinantly produced uronic acid oxidase from Citrus sinensis. Enzymatic oxidation with uronic acid oxidase afforded 95 % yield in 72 hours which is considerably higher than yields previously achieved using a glucooligosaccharide oxidase from Sarocladium strictum.
View Article and Find Full Text PDFFront Microbiol
January 2025
School of Science, Engineering and Environment, University of Salford, Salford, United Kingdom.
The microbiota within the guts of insects plays beneficial roles for their hosts, such as facilitating digestion and extracting energy from their diet. The African palm weevil (APW) lives within and feeds on the high lignin-containing trunk of palm trees; therefore, their guts could harbour a large community of lignin-degrading microbes. In this study, we aimed to explore the bacterial community within the gut of the APW larvae, specifically with respect to the potential for lignin degradation in various gut segments as a first step to determining the viability of mining bacterial lignin-degrading enzymes for the bioconversion of lignocellulosic biomass to biofuels and biomaterials.
View Article and Find Full Text PDFACS Environ Au
January 2025
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-Ku, Kobe 657-8501, Japan.
Pretreatment of lignocellulosic biomass is crucial yet challenging for sustainable energy production. This study focuses on enhancing enzymatic accessibility of cellulose in oil palm empty fruit bunches by optimizing pretreatment parameters to improve glucose and ethanol yields while reducing fermentation inhibitors. It evaluates the impact of maleic acid concentrations on biorefinery processes.
View Article and Find Full Text PDFBiofuels derived from renewable and sustainable lignocellulosic biomass, such as switchgrass, offer a promising means to limit greenhouse gas emissions. However, switchgrass grown under drought conditions contains high levels of chemical compounds that inhibit microbial conversion to biofuels. Fermentation of drought switchgrass hydrolysates by engineered and generates less ethanol than fermentation of hydrolyzed switchgrass from an average rainfall year.
View Article and Find Full Text PDFBioresour Technol
January 2025
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, No. 2 Xuelin Road, Nanjing 210097, China. Electronic address:
Lignocellulosic biomass is the most abundant form of biomass available for fuel production, serving as the fourth leading energy source globally. However, inhibitors generated during pretreatment processes often hinder fermentation performance and conversion efficiency. In this study, we developed an enhanced computer-assisted enzyme cocktail strategy (ComEC 2.
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