Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered lipolytica, the isomaltulose production reached the highest (102.6 g L¹) at flask level with pretreated cane molasses of 350 g L¹ and corn steep liquor of 1.0 g L¹. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L¹) was achieved with 0.96 g g¹ yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L¹). Additionally, the lipids comprised of 94.29% C and C fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6480463 | PMC |
| http://dx.doi.org/10.3390/molecules24071228 | DOI Listing |
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