AI Article Synopsis
Article Abstract
A small, consumable-free, low-power, ultra-high-speed comprehensive GC×GC system consisting of microfabricated columns, nanoelectromechanical system (NEMS) cantilever resonators for detection, and a valve-based stop-flow modulator is demonstrated. The separation of a highly polar 29-component mixture covering a boiling point range of 46 to 253 °C on a pair of microfabricated columns using a Staiger valve manifold in less than 7 seconds, and just over 4 seconds after the ensemble holdup time is demonstrated with a downstream FID. The analysis time of the second dimension was 160 ms, and peak widths in the second dimension range from 10-60 ms. A peak capacity of just over 300 was calculated for a separation of just over 6 s. Data from a continuous operation testing over 40 days and 20 000 runs of the GC×GC columns with the NEMS resonators using a 4-component test set is presented. The GC×GC-NEMS resonator system generated second-dimension peak widths as narrow as 8 ms with no discernable peak distortion due to under-sampling from the detector.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c9lc00027e | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Elastic ribbons in bubble columns: When elasticity, capillarity, and gravity govern equilibrium configurations.
Phys Rev E
August 2024
Université de Strasbourg, CNRS, Institut Charles Sadron UPR22, F-67000 Strasbourg, France.
Taking advantage of the competition between elasticity and capillarity has proven to be an efficient way to design structures by folding, bending, or assembling elastic objects in contact with liquid interfaces. Elastocapillary effects often occur at scales where gravity does not play an important role, such as in microfabrication processes. However, the influence of gravity can become significant at the desktop scale, which is relevant for numerous situations including model experiments used to provide a fundamental physics understanding, working at easily accessible scales.
View Article and Find Full Text PDFComputational Pulsatile Flow and Efficiency Analysis of Biocompatible Microfluidic Artificial Lungs for Different Fiber Configurations.
J Biomech Eng
August 2024
Mechanical Engineering Department, Boğaziçi University, Bebek, İstanbul 34342, Turkey.
Average-sized microfluidic artificial lungs consisting of rows and columns of fiber bundles with different column to row aspect ratios (AR) are numerically analyzed for flow characteristics, maximum gas transfer performance, minimum pressure drop, and proper wall shear stress (WSS) values in terms of biocompatibility. The flow is fully laminar and assumed to be incompressible and Newtonian. The transport analysis is performed using a combined convection-diffusion model, and the numerical simulations are carried out with the finite element method.
View Article and Find Full Text PDFUse of a Microfluidic Platform To Evaluate and Predict Reagent Performance in Microtiter Plate-Based Immunoassays.
Anal Chem
February 2024
Merck & Co., Inc., West Point, Pennsylvania 19486, United States.
Selection and characterization of antibodies are critically important in establishing robust immunoassays to support the development efforts of vaccines. Plate-based ELISA can be time- and resource-intensive to select initial antibody clones or characterize downstream resupply lots while providing limited information regarding the binding characteristics of the antibodies beyond concentration-response curves. This work applied the microfluidic Gyrolab to holistically evaluate immunoassay reagents through analyses of concentration-response curves as well as antibody-antigen interactions visualized in column images and affinity estimates.
View Article and Find Full Text PDFToward 3D printed microfluidic artificial lungs for respiratory support.
Lab Chip
February 2024
ECLS Laboratory, Department of Surgery, University of Michigan, Ann Arbor, MI 48109, USA.
Microfluidic artificial lungs (μALs) are a new class of membrane oxygenators. Compared to traditional hollow-fiber oxygenators, μALs closely mimic the alveolar microenvironment due to their size-scale and promise improved gas exchange efficiency, hemocompatibility, biomimetic blood flow networks, and physiologically relevant blood vessel pressures and shear stresses. Clinical translation of μALs has been stalled by restrictive microfabrication techniques that limit potential artificial lung geometries, overall device size, and throughput.
View Article and Find Full Text PDFHigh-Throughput Liquid Chromatographic Analysis Using a Segmented Flow Injector with a 1 s Cycle Time.
Anal Chem
November 2023
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
High-throughput screening (HTS) workflows are revolutionizing many fields, including drug discovery, reaction discovery and optimization, diagnostics, sensing, and enzyme engineering. Liquid chromatography (LC) is commonly deployed during HTS to reduce matrix effects, distinguish isomers, and preconcentrate prior to detection, but LC separation time often limits throughput. Although subsecond LC separations have been demonstrated, they are rarely utilized during HTS due to limitations associated with the speed of common autosamplers.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!