Fluoroquinolones are important therapeutics in human and veterinary medicine. This study aimed to retrospectively analyse sedimentation dusts from intensive-livestock-farming barns for fluoroquinolones and investigate the association between resistant Escherichia coli and the detected drugs. Sedimentation-dust samples (n = 125) collected (1980-2009) at 14 barns of unknown-treatment status were analysed by HPLC and tandem-mass spectroscopy to detect enrofloxacin, ciprofloxacin, marbofloxacin, and difloxacin. Recent microbiological data were included to investigate the relationship between fluoroquinolone presence and fluoroquinolone-resistant E. coli. Fifty-nine dust samples (47%) from seven barns contained fluoroquinolone residues. Up to three different fluoroquinolones were detected in pig and broiler barns. Fluoroquinolone concentrations ranged from 10-pg/mg to 46-ng/mg dust. Fluoroquinolone-resistant E. coli were isolated from four barns. Of all the dust samples, 22% contained non-susceptible isolates. Non-susceptible isolate presence in the dust was significantly associated (p = 0.0283) with detecting the drugs, while drug detection increased the odds (4-fold) of finding non-susceptible E. coli (odds ratio = 3.9877, 95% CI: 1.2854-12.3712). This retrospective study shows that fluoroquinolone usage leads to dust contamination. We conclude that farmers and animals inhale/swallow fluoroquinolones and fluoroquinolone-resistant bacteria due to drug application. Furthermore, uncontrolled drug emissions via air exhausted from the barns can be assumed.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435704 | PMC |
| http://dx.doi.org/10.1038/s41598-019-41528-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
BioBERT based text mining for incorporating prior knowledge in the inference of genetic network models.
Comput Biol Med
January 2025
Health Innovation and Transformation Centre, Federation University, Victoria, 3842, Australia; BioThink, Queensland, 4020, Australia.
Reconstruction of Gene Regulatory Networks (GRNs) is essential for understanding gene interactions, their impact on cellular processes, and manifestation of diseases, including drug discovery. Among various mathematical and dynamic models used for GRN reconstruction, S-system model, comprising non-linear differential equations, is widely utilised to capture the behaviour of complex biological systems with non-linear and time-dependent interactions. However, as the network size increases, computational demand for network inference grows due to a greater number of estimation parameters, significantly impacting the performance of optimisation algorithms.
View Article and Find Full Text PDFIsolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli.
BMC Res Notes
January 2025
Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt.
Objectives: The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination.
View Article and Find Full Text PDFCarboxymethyl hemicellulose hydrogel as a fluorescent biosensor for bacterial and fungal detection with DFT and molecular docking studies.
Sci Rep
January 2025
Cellulose and Paper Department, National Research Centre, 33 El Bohouth Str, P.O. 12622, Dokki Giza, Egypt.
A new method was developed to quickly produce carboxymethyl hemicellulose (CM-Hemi) and fluorescent nitrogen-doped carbon dots (N-CDs) from sugarcane bagasse (SB). These materials were then combined with calcium chloride (CaCl₂) to create hydrogel sensors with antibacterial and antifungal properties. The CM-Hemi@Ca-N-CDs hydrogel was effective against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria compared to CM-Hemi@Ca which give no antibacterial activity.
View Article and Find Full Text PDFIsatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets.
Vet Q
December 2025
Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores ( < 0.
View Article and Find Full Text PDFThe proteome is a terminal electron acceptor.
Proc Natl Acad Sci U S A
January 2025
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.
Microbial metabolism is impressively flexible, enabling growth even when available nutrients differ greatly from biomass in redox state. , for example, rearranges its physiology to grow on reduced and oxidized carbon sources through several forms of fermentation and respiration. To understand the limits on and evolutionary consequences of this metabolic flexibility, we developed a coarse-grained mathematical framework coupling redox chemistry with principles of cellular resource allocation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!