Saturated aliphatic acids (C to C) and n-alkyl formate, acetate, propionate and butyrate esters (C to C) were evaluated to determine the minimum inhibitory concentrations (MIC) necessary to inhibit the growth of Clostridium botulinum 62A in a bacteriological medium. The C to C and C to C acids and esters were relatively inactive (MIC > 200 μg/ml). The C to C acids exhibited some antibotulinal acitivity (MIC = 100 μg/ml), whereas C to C esters were substantially more inhibitory. The n-alkyl formates, acetates and propionates having C to C groups gave the highest inhibition, with MIC's of 3.1, 6.3 and 6.3 μg/ml, respectively. The maximum inhibition for the butyrates (MIC = 12.4 μg/ml) was associated with C to C alkyl groups. A mathematical relationship between activity and alkyl group chain length was derived.
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