This study aims to produce transgenic cattle expressing lysine-rich polypeptide in milk by somatic cell nuclear transfer. Lysine is the first limiting amino acid in cereal crops. Lysine deficiency leads to fatigue, nausea and reproductive disorders. In this study, transgenic cells with lysine-rich cDNA (β-casein, αS2-casein and lactotransferrin cDNA) were used as donor cells to produce transgenic cattle by somatic cell nuclear transfer. The transgene integration and expression in transgenic cattle were examined using western blotting, Southern blotting, PCR and RT-PCR. Our results showed that the lysine-rich gene was successfully integrated into the bovine genome and expressed in the mammary gland of transgenic cattle during lactation. The milk lysine concentrations of three transgenic cattle were higher than those of non-transgenic controls. The production of transgenic cattle expressing lysine-rich polypeptides in milk may provide a large-scale and cost-effective resource for lysine-rich products.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11248-019-00124-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genetically modified chickens as bioreactors for protein-based drugs.
Front Genome Ed
January 2025
Frontiers Science Center for Molecular Design Breeding (MOE), State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, China.
Protein drug production encompasses various methods, among which animal bioreactors are emerging as a transgenic system. Animal bioreactors have the potential to reduce production costs and increase efficiency, thereby producing recombinant proteins that are crucial for therapeutic applications. Various species, including goats, cattle, rabbits, and poultry, have been genetically engineered to serve as bioreactors.
View Article and Find Full Text PDFGeneration of Codon-Optimized Fad3 Gene Transgenic Bovine That Produce More n-3 Polyunsaturated Fatty Acids.
Animals (Basel)
January 2025
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock (R2BGL), Inner Mongolia University, 24 Zhaojun Rd., Hohhot 010070, China.
Polyunsaturated fatty acids (PUFAs) such as linoleic acid (18:2, n-6) and α-linolenic acid (18:3, n-3) are essential for the growth, development, and well-being of mammals. However, most mammals, including humans, cannot synthesize n-3 and n-6 PUFAs and these must be obtained through diet. The beneficial effect of converting n-6 polyunsaturated fatty acids (n-6 PUFAs) into n-3 polyunsaturated fatty acids (n-3 PUFAs) has led to extensive research on the flax fatty acid desaturase 3 () gene, which encodes fatty acid desaturase.
View Article and Find Full Text PDFControlled Dynamic Microfluidic Culture of Murine, Bovine, and Human Embryos Improves Development: Proof-of-Concept Studies.
Cells
December 2024
Department of Obstetrics and Gynecology, University of Michigan, 1500 E. Medical Center Dr., Ann Arbor, MI 48109-0617, USA.
Classical preimplantation embryo culture is performed in static fluid environments. Whether a dynamic fluid environment, like the fallopian tube, is beneficial for embryo development remains to be determined across mammalian species. Objectives of these proof-of-concept studies were to determine if controllable dynamic microfluidic culture would enhance preimplantation murine, bovine, and human embryo development compared to static culture.
View Article and Find Full Text PDFCommercial perspectives: Genome editing as a breeding tool for health and well-being in dairy cattle.
JDS Commun
November 2024
Department of Preventive Veterinary Medicine and Animal Reproduction, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, 14884-900, Brazil.
Genome editing is the latest breeding tool capable of accelerating the rate of genetic improvement for health and well-being traits in food animals. It enables the introduction of beneficial alleles within a single generation, including those that are of low frequency or absent in the population, while effectively bypassing linkage drag. For the dairy industry, genome editing can be used to make rapid genetic improvements that are precise, efficient, and transgene-free for functional traits that are not practically addressed without disrupting conventional breeding goals for overall economic merit based on genomic selection.
View Article and Find Full Text PDFCharacterization of NFDQ1 in Cryptosporidium parvum.
Parasit Vectors
October 2024
State Key Laboratory for Animal Disease Control and Prevention Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Article Synopsis
- - Cryptosporidium spp. are zoonotic parasites that cause diarrhea in both humans and animals, with Cryptosporidium parvum leading to severe symptoms in calves, while C. bovis and C. ryanae typically do not exhibit illness.
- - Researchers performed comparative genomic analysis, revealing differences in genes related to a secretory protein family (NFDQ), potentially linked to host range and pathogenicity of these parasites, and further investigated the specific function of one such protein, NFDQ1.
- - Using CRISPR/Cas9 technology, scientists successfully tagged and created knockout strains for NFDQ1, confirming its presence through various assays, which demonstrated that NFDQ1 is expressed during both a
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!