Antioxidant Potential of Aqueous Leaves Extract in Modulating Restraint Stress-Induced Changes in Rat's Liver.

Introduction: This study was carried out to evaluate the antioxidant potential of crude extract of leaves and its active constituents as treatment against restraint stress in rat's liver.

Methods: For this purpose, male albino Wistar rats were treated with crude extract of leaves and its alkaloid and flavonoid fractions both before and after 6 h of acute restraint stress. Prooxidant status of rat liver was assessed by determining the levels of thiobarbituric acid reactive substances, reduced glutathione, alkaline phosphatase, alanine transaminase, aspartate aminotransferase, and the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST).

Results: Six hours of restraint stress generated oxidative stress in rat's liver resulted in a significant rise in the level of the aforementioned liver enzymes. On the other hand, SOD, CAT, and GST enzymatic activities showed a significant decline in their level. The administration of crude leaves extract, both before and after stress exposure, significantly prevented the rise in the level of liver enzymes and reverted the activities of studied biochemical parameters toward their normal control values. However, the reversion was found to be more prominent in after-stress group.

Conclusion: The aforementioned results highlight the significant antioxidant potential of extracts. On the basis of our study, we suggest the possible use of leaves extract as a nutritional supplement for combating oxidative stress induced damage.