Vibrio fluvialis is considered as a human pathogen in developing countries. This bacterium is widely distributed in seawater and harbors that contains traces of salt. V. fluvialis can cause human enteritis and diarrhea, which has broken out at a global scale. Lipopolysaccharide (LPS) is a key bacterial antigen used to classify V. fluvialis serogroups. In this research, phage display technology was adopted to isolate nanobodies from a naïve phage library by using LPS as the target antigen. The isolated nanobody was tested in LPS ELISA and bacterial enzyme-linked immunosorbent assay Nanobody V23 had a high affinity toward the pathogen and was utilized to synthesize immunomagnetic beads for the enrichment of V. fluvialis. The capture efficiency of the immunomagnetic beads against V. fluvialis was 90.7 ± 3.2% (N = 3) through the plate-counting method. We generated a high-affinity nanobody against LPS from V. fluvialis and developed a rapid method of enriching V. fluvialis by using immunomagnetic beads.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2019.03.104 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microfluidic Integration of Magnetically Functionalized Microwires for Flow Cytometry Protein Quantification.
Materials (Basel)
January 2025
Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada.
A novel approach to protein quantification utilizing a microfluidic platform activated by a magnetic assembly of functionalized magnetic beads around soft magnetic capture centers is presented. Functionalized magnetic beads, known for their high surface area and facile manipulation under external magnetic fields, are injected inside microfluidic channels and immobilized magnetically on the surface of glass-coated soft magnetic microwires placed along the symmetry axis of these channels. A fluorescent (Cy5) immunomagnetic sandwich ELISA is then performed by sequentially flowing the sample and all necessary reagents in the microfluidic channels.
View Article and Find Full Text PDFFluorescence immunochromatographic assay for deoxynivalenol using immunomagnetic bead purification.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
February 2025
College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Deoxynivalenol (DON) contaminates various complex matrices, necessitating straightforward, effective cleanup and precise detection methods. This study employed immunomagnetic beads for sample purification and utilized a competitive time-resolved fluoro-immuno-chromatographic assay to achieve quantitative detection of DON in corn and its by-products. The limits of detection and quantification were 104 μg/kg and 243 μg/kg, respectively.
View Article and Find Full Text PDFCorrection: Hedgehog-inspired immunomagnetic beads for high-efficient capture and release of exosomes.
J Mater Chem B
January 2025
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.
Correction for 'Hedgehog-inspired immunomagnetic beads for high-efficient capture and release of exosomes' by Jia Cheng , 2022, , 4059-4069, https://doi.org/10.1039/D2TB00226D.
View Article and Find Full Text PDFElectrochemical Magnetic Immunoassay for the Determination of the Fish Allergen β-Parvalbumin.
Biosensors (Basel)
December 2024
REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4249-015 Porto, Portugal.
β-parvalbumin (β-PV) is the primary fish allergen responsible for most allergic reactions in individuals sensitive to fish. To ensure food safety, a sandwich-based magnetic immunoassay was developed using maleimide-functionalized magnetic beads (NH-MBs). Specific anti-β-PV antibodies were immobilized on these MBs, and a screen-printed carbon electrode was employed as the electrochemical transducer.
View Article and Find Full Text PDFDirect comparison of an ultrasensitive real-time PCR assay with droplet digital PCR for the detection of hotspot mutations in primary tumors, plasma cell-free DNA and paired CTC-derived gDNAs.
Front Oncol
December 2024
Analysis of Circulating Tumor Cells, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece.
Article Synopsis
- The study compares the effectiveness of ultrasensitive real-time PCR and droplet digital PCR (ddPCR) in detecting specific mutations associated with breast cancer in primary tumors and liquid biopsy samples.
- The research involved analyzing genetic material from 42 tumor samples and 29 plasma samples from patients with ER+ metastatic breast cancer, as well as samples from healthy donors.
- Results showed that both methods provided similar detection rates for certain mutations in tumor samples, with ultrasensitive real-time PCR performing better in plasma-cfDNA samples, indicating potential for non-invasive testing in cancer management.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!