AI Article Synopsis
- - A new method for labeling glycosaminoglycan (GAG) oligosaccharides was developed using thia-Michael addition and deuterium incorporation at both ends of the molecules.
- - The technique works well even at a small scale, making it suitable for analyzing heparin, a type of GAG.
- - Importantly, this labeling method does not interfere with the function of key enzymes like heparinase I and sulfatase HSulf-2.
Article Abstract
A novel double labelling of glycosaminoglycans (GAG) oligosaccharides by thia-Michael addition and deuterium incorporation at the non-reducing and reducing ends, respectively, was introduced. This was demonstrated to be both compatible with the heparin microgram scale and amenable for mass spectrometry analysis, without impairing enzymatic activities such as heparinase I and sulfatase HSulf-2.
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| http://dx.doi.org/10.1039/c9cc00254e | DOI Listing |
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