AI Article Synopsis
- Researchers studied how FGF2 expression in cumulus cells is regulated by FSH and substances from oocytes during in vitro maturation (IVM) in bovine models.
- The study found that FGF2, when added to IVM, sped up meiotic progress, made it harder to dissociate cumulus cells, and reduced cell death among those cells.
- This research highlights the role of oocytes in regulating FGF2 expression and suggests that FGF2 may enhance the survival and quality of cumulus cells during the maturation process.
Article Abstract
Purpose: We first assessed regulation of FGF2 expression in cumulus cells by FSH and oocyte-secreted factors during in vitro maturation (IVM). Then, we tested the hypothesis that FGF2 regulates meiotic progression, cumulus expansion, and apoptosis in cumulus-oocyte complexes (COC) undergoing IVM.
Methods: In vitro maturation of bovine COC was utilized as a model to assess regulation of FGF2 expression by FSH and oocyte-secreted factors (via microsurgical removal of the oocyte), as well as effects of graded doses of FGF2 on meiotic progression, degree of cumulus expansion, dissociation of cumulus cells, and cumulus cells apoptosis. Expression of genes regulating functional endpoints altered by FGF2 treatment was assessed in cumulus cells by real-time PCR. Cultures were replicated 4-5 times and effects of treatments were tested by ANOVA.
Results: FGF2 mRNA expression was increased by FSH and oocyte-secreted factors during IVM. Addition of FGF2 to the IVM medium advanced meiosis resumption, decreased the ease with which cumulus cells were dissociated, and inhibited cumulus cells apoptosis. Decreased cumulus dissociation was accompanied by decreased expression of TNFAIP6.
Conclusions: This is the first study showing that FGF2 expression is regulated by the oocyte in cumulus cells. Moreover, we report novel effects of FGF2 on cumulus cell survival and extracellular matrix (ECM) quality during IVM that may favor acquisition of developmental competence and suggest physiological roles during the final steps of COC differentiation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541720 | PMC |
| http://dx.doi.org/10.1007/s10815-019-01436-7 | DOI Listing |
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