Hydrogen peroxide (HO) quantification in biomedicine is valuable as inflammation biomarker but also in assays employing enzymes that generate or consume HO linked to a specific biomarker. Optical HO detection is typically performed through peroxidase-coupled reactions utilizing organic dyes that suffer, however, from poor stability/reproducibility and also cannot be employed in situ in dynamic complex cell cultures to monitor HO levels in real-time. Here, we utilize enzyme-mimetic CeO nanocrystals that are sensitive to HO and study the effect of HO presence on their electronic and luminescent properties. We produce and dope with Eu these particles in a single-step by flame synthesis and directly deposit them on Si and glass substrates to fabricate nanoparticle layers to monitor in real-time and in situ the HO concentrations generated by Streptococcus pneumoniae clinical isolates. Furthermore, the small CeO:Eu nanocrystals are combined in a single-step with larger, non-responsive YO:Tb nanoparticles during their double-nozzle flame synthesis to engineer hybrid luminescent nanoaggregates as ratiometric robust biosensors. We demonstrate the functionality of these biosensors by monitoring their response in the presence of a broad range of HO concentrations in vitro from S. pneumoniae, highlighting their potential for facile real-time HO detection in vitro in cell cultures.
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| http://dx.doi.org/10.1016/j.bios.2019.03.012 | DOI Listing |
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