AI Article Synopsis
- This study presents a simple and cost-effective method to detect harmful microbial pathogens in drinking water using silver nanoparticles that interact with urease.
- In the presence of pathogens, these silver nanoparticles bind to the bacteria, which then causes urease to increase the solution's pH.
- A color-changing dye responds to the pH change, allowing clear detection of bacterial contamination at levels as low as 102 cells per milliliter.
Article Abstract
Herein, we demonstrate a facile and economic approach for colorimetric detection of microbial pathogens in drinking water, employing silver-urease interactions. In the presence of harmful pathogens, receptor coated silver nanoparticles (AgNPs) preferentially bind to the bacterial surface and urease catalytically elevates the pH of the solution, which is sensed by a pH responsive chromogenic dye. The assay demarcates bacterial contamination levels up to 102 cells mL-1 in a field-friendly method.
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| http://dx.doi.org/10.1039/c9cc00225a | DOI Listing |
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