AI Article Synopsis
- The KF-1 monoclonal antibody targets a specific component of the lamina densa in the basement membrane of human skin and is classified as an IgG3 type.
- Its binding properties were analyzed, revealing it does not interact with staphylococcal protein A and is expressed in two squamous carcinoma cell lines, TR131 and TR146.
- Techniques like immunofluorescence and immunoblot analysis confirmed that the antigen is a cell surface protein sensitive to Triton X-100, and it identifies a 72 kD polypeptide in both TR131 and human keratinocyte extracts.
Article Abstract
The KF-1 monoclonal antibody has been shown to identify a noncollageneous component of the lamina densa of the basement membrane zone of human skin. In the present work, the monoclonal antibody and the antigen were further characterized. KF-1 monoclonal antibody is an IgG3 immunoglobulin with no binding to staphylococcal protein A. Two squamous carcinoma cell lines--namely, TR131 and TR146--quantitatively express the antigen. Immunofluorescence techniques showed that the antigen is a cell surface antigen, is sensitive to Triton X-100 extraction, and is concentrated in cell areas from which actin fibers are excluded. Immunoblot analysis showed that this monoclonal antibody identifies a 72 kD polypeptide present in TR131 cell extract as well as in cultured human keratinocyte cell extract.
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| http://dx.doi.org/10.1111/1523-1747.ep12523584 | DOI Listing |
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