Soy1 (IAVPTGVA) and Lup1 (LTFPGSAED), two peptides from soybean and lupin protein hydrolysis, have been singled out as dipeptidyl peptidase IV (DPP-IV) activity inhibitors in different model systems. However, their activity is affected by their instability toward intestinal proteases. Here, an innovative strategy based on nanogels was developed in order to increase both their stability and antidiabetic properties through encapsulation into the RADA16 peptide. The nanogel formation was stimulated by a solvent-triggered approach, allowing us to produce stable nanogels ( G' = 1826 Pa, stress-failure ≥50 Pa) with shear-thinning propensity. ThT binding assay, and ATR-FTIR spectroscopy experiments showed that nanogels self-aggregated into stable cross-β structures providing higher resistance against proteases (ex vivo experiments) and increased bioavailability of Soy1 and Lup1 peptides (in situ experiments on Caco-2 cells). Hence, this simple and harmless nanotechnological approach could be a key-step in making innovative nanomaterials for nutraceuticals delivering.
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http://dx.doi.org/10.1021/acs.jafc.8b07264 | DOI Listing |
Heliyon
July 2024
Department of Plant Pathology, University of Georgia, Tifton, GA, 31793, USA.
The resistance () gene family in plants is a vital component of the plant defense system, enabling host resistance against pathogens through interactions with pathogen effector proteins. These R genes often encode nucleotide-binding (NB-ARC or N) and leucine-rich-repeat (LRR or L) domains, collectively forming the NLR protein family. The NLR proteins have been widely explored in crops from and , but limited studies are available for crops in other families, including .
View Article and Find Full Text PDFAnimals (Basel)
December 2024
School of Molecular and Life Sciences, Curtin University, 1 Turner Avenue, Bentley, WA 6102, Australia.
We investigated the effect of feed deprivation for 45 days on the growth, immunity, and health of 0marron () initially fed for 110 days on various protein sources including fishmeal (FM), poultry by-product meal (PBM), black soldier fly meal (BSFM), soybean meal (SBM), lupin meal (LM), and tuna hydrolysate. The marron were weighed and sacrificed immediately after feeding stopped (day 0) and at days 15, 30, and 45 after the feed deprivation trial commenced. Total haemolymph count, differential haemocyte count, lysozyme activity, protease activity, total bacterial count in the digestive tract, and organosomatic indices were analysed.
View Article and Find Full Text PDFCurr Res Food Sci
November 2024
College of Food and Bioengineering, Xihua University, Chengdu, 610039, China.
Anal Chim Acta
December 2024
Centro de Investigação em Química da Universidade do Porto/ Instituto de Ciências Moleculares (CIQUP/IMS), Faculdade de Ciências da Universidade do Porto, Departamento de Química e Bioquímica Rua do Campo Alegre 687, 4169-007, Porto, Portugal. Electronic address:
Hum Immunol
November 2024
Department of Allergology, National Medical Institute of the Ministry of the Interior and Administration, Warsaw, Poland. Electronic address:
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