Clinical trials have shown that administering heparin during the luteal phase has beneficial effects on implantation and live birth rates. Heparin exerts direct effects on decidual human endometrial stromal cells (HESCs), which are independent of its anticoagulant effect. However, the accurate effects of heparin on the decidualization process remain unidentified. Here, we demonstrate that HESCs become dramatically resistant to oxidative stress upon decidualization, and we hypothesize a possible direct action of heparin on the decidualization of HESCs, which would lead to improved implantation. To test this hypothesis, we established primary HESC cultures and propagated them, and then we decidualized confluent cultures with 8-bromo-cAMP, with medroxyprogesterone acetate, and with or without heparin. We treated the cells with hydrogen peroxide (HO) as a source of reactive oxygen species (ROS). Adding heparin to decidualized HESCs induced prolactin secretion. Decidualized HESCs treated with heparin were prevented from undergoing apoptosis induced by oxidative stress. Heparin induced nuclear accumulation of the forkhead transcription factor FOXO1 and expression of its downstream target, the ROS scavenger superoxide dismutase 2. These results demonstrate that heparin-treated decidualized HESCs acquired further resistance to oxidative stress, suggesting that heparin may improve the implantation environment.
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http://dx.doi.org/10.1007/s00795-019-00220-x | DOI Listing |
Inflammopharmacology
December 2024
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore Campus, Lahore, 5400, Pakistan.
Rheumatoid arthritis is an autoimmune disorder affecting multiple joints and requires lifelong treatment. Present study was designed to formulate Esculin-loaded chitosan nanoparticles (ENPs) and evaluation of its anti-inflammatory and anti-arthritic action. The acute toxicity study of ENPs was also performed.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
December 2024
Department of Agronomy, Faculty of Agricultural Sciences, SGT University, Gurugram, India.
Chromium (Cr) is an ever-present abiotic stress that negatively affects crop cultivation and production worldwide. High rhizospheric Cr concentrations inhibit nutrients uptake and their translocation to aboveground parts, thus can affect the growth and development of crop plants. This experiment was designed to evaluate the effects of sole and combined zinc-lysine and iron-lysine applications on photosynthetic efficacy, antioxidative defense, oxidative stress, and nutrient uptake and translocation under Cr stress.
View Article and Find Full Text PDFBiol Trace Elem Res
December 2024
Department of Biochemistry, Faculty of Pharmacy, University of Sadat City (USC), Menoufia, Egypt.
Metabolic syndrome during menopause can lead to diabetes, cardiovascular problems, and increased mortality rates. Hormone replacement therapy is recommended to manage climacteric complications, but it has serious adverse effects. This study, therefore, investigated the potential of supplementing some minerals, vitamins, and natural products like boric acid, magnesium, vitamin D3, and extra virgin olive oil on metabolic status of menopausal ovariectomized rats.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
December 2024
Life Sciences and Bioengineering Center, Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA, USA.
Transcriptomics is a powerful approach for functional genomics and systems biology, yet it can also be used for genetic part discovery. Here, we derive constitutive and light-regulated promoters directly from transcriptomics data of the basidiomycete red yeast Xanthophyllomyces dendrorhous CBS 6938 (anamorph Phaffia rhodozyma) and use these promoters with other genetic elements to create a modular synthetic biology parts collection for this organism. X.
View Article and Find Full Text PDFActa Diabetol
December 2024
Department of Endocrinology, General Hospital of Central Theater Command, Wuhan, Hubei, People's Republic of China.
Aims: There is a potential association between oxidative stress and the development of diabetic kidney disease (DKD). The Oxidative Balance Score (OBS), derived from dietary and lifestyle factors, acts as a comprehensive marker of oxidative stress. Research examining the relationship between OBS and DKD is scarce.
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