Microalga Scenedesmus vacuolatus exhibited maximum growth, protein and carbohydrate contents at 10.0 mM concentration of nitrate, 1.0 mM of glutamate nitrogen and at C/N ratio (12 mM acetate+10 mM nitrate). However, these cell constituents showed the highest values in the C+N grown cells, but the lipid content was found to be the highest glutamate grown cells. FTIR analysis of Lipid/Carbohydrate and Lipid/Protein ratio and flow cytometric analysis of neutral lipids revealed higher lipid content in the glutamate grown cells than in the nitrogen starved, nitrate and C+N grown cells. The nitrate reductase activity was the highest in the C+ N grown cells and the lowest activity was found in the glutamate grown cells. A corollary of these results suggested that suppression of nitrogen assimilatory system, whether by glutamate or by nitrogen deprivation, was the most suitable physiological condition for enhanced lipid synthesis and biofuel production in microalgal cells.
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